Nanogels fabricated by lysozyme and sodium carboxymethyl cellulose for 5-fluorouracil controlled release

被引：106
作者：
Zhu, Kunkun [1 ,2 ,3 ]
Ye, Ting [1 ,2 ]
Liu, Jinjin [1 ,2 ]
Peng, Zheng [3 ]
Xu, Shasha [1 ,2 ]
Lei, Jieqiong [1 ,2 ]
Deng, Hongbing [1 ,2 ]
Li, Bin [1 ,2 ]
机构：
[1] Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
[3] Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2013年 / 441卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Lysozyme; CMC; Electrostatic attraction; Nanogels; Drug delivery; DELIVERY-SYSTEMS; CARBON-DIOXIDE; CHITOSAN; NANOPARTICLES; DENATURATION; DEXTRAN; GELATION; CARRIER; CASEIN;
D O I：
10.1016/j.ijpharm.2012.10.022
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Lysozyme (Ly) and sodium carboxymethyl cellulose (CMC) were used to fabricate nanogels by a convenient method without using any chemical treatment except simple heating to achieve the denaturation temperature of Ly. The prepared nanogels were characterized by dynamic laser scattering (DLS), rheological analysis, transmission electron microscopy (TEM), field emission scanning electron microscope (FE-SEM) and X-ray photoelectron spectroscopy (XPS). The nanogels are of spherical shape with average hydrodynamic diameter of 241 nm and the swelling ratio of nanogels is about 5. Then 5-fluorouracil was used as a model drug to investigate the entrapment efficiency and release ability in nanogels. It turned out to be that the release in simulated gastric fluid (SGF) was more slowly compared with that in simulated intestinal fluid (SIF), which could protect the 5-Fu in stomach and ensure it released in intestines. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：721 / 727
页数：7
相关论文
共 37 条
[1]   THE 3 STATES OF GLOBULAR-PROTEINS - ACID DENATURATION [J].
ALONSO, DOV ;
DILL, KA ;
STIGTER, D .
BIOPOLYMERS, 1991, 31 (13) :1631-1649
[2]   3-Diethylaminopropyl-bearing glycol chitosan as a protein drug carrier [J].
Baik, Hye Jung ;
Oh, Nam Muk ;
Oh, Young Taik ;
Yoo, Na Young ;
Park, So Young ;
Oh, Kyung Taek ;
Youn, Yu Seok ;
Lee, Eun Seong .
COLLOIDS AND SURFACES B-BIOINTERFACES, 2011, 84 (02) :585-590
[3]   COMPARISON OF SELF-ASSEMBLED MONOLAYERS ON GOLD - COADSORPTION OF THIOLS AND DISULFIDES [J].
BAIN, CD ;
BIEBUYCK, HA ;
WHITESIDES, GM .
LANGMUIR, 1989, 5 (03) :723-727
[4]  
Cai X., 2011, INT J PHARMACEUT, V69, P31
[5]   Charged nanoparticles as protein delivery systems: A feasibility study using lysozyme as model protein [J].
Cal, Cuifang ;
Bakowsky, Udo ;
Rytting, Erik ;
Schaper, Andreas K. ;
Kissel, Thomas .
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2008, 69 (01) :31-42
[6]   Food protein-based materials as nutraceutical delivery systems [J].
Chen, LY ;
Remondetto, GE ;
Subirade, M .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2006, 17 (05) :272-283
[7]   Macroporous chitosan/carboxymethylclellulose blend membranes and their application for lysozyme adsorption [J].
Chen, X ;
Liu, JH ;
Feng, ZC ;
Shao, ZZ .
JOURNAL OF APPLIED POLYMER SCIENCE, 2005, 96 (04) :1267-1274
[8]   Direct purification of lysozyme from chicken egg white using weak acidic polyacrylonitrile nanofiber-based membranes [J].
Chiu, Hsien-Tang ;
Lin, Jian-Min ;
Cheng, Tai-Hong ;
Chou, Shin-Ying ;
Huang, Cheng-Chiang .
JOURNAL OF APPLIED POLYMER SCIENCE, 2012, 125 :E616-E621
[9]   Green Preparation Process, Characterization and Antitumor Effects of Doxorubicin-BSA-Dextran Nanoparticles [J].
Deng, Wei ;
Li, Juan ;
Yao, Ping ;
He, Fen ;
Huang, Chong .
MACROMOLECULAR BIOSCIENCE, 2010, 10 (10) :1224-1234
[10]   Tumor-targeted drug delivery using MR-contrasted docetaxel - Carboxymethylcellulose nanoparticles [J].
Ernsting, Mark J. ;
Foltz, Warren D. ;
Undzys, Elijus ;
Tagami, Tatsuaki ;
Li, Shyh-Dar .
BIOMATERIALS, 2012, 33 (15) :3931-3941
1234