A Transgenic Mouse Marking Live Replicating Cells Reveals In Vivo Transcriptional Program of Proliferation

被引：53

作者：

Klochendler, Agnes ^{[1
]}

Weinberg-Corem, Noa ^{[3
]}

Moran, Maya ^{[1
]}

Swisa, Avital ^{[3
]}

Pochet, Nathalie ^{[4
]}

Savova, Virginia ^{[4
]}

Vikesa, Jonas ^{[5
]}

Van de Peer, Yves ^{[6
]}

Brandeis, Michael ^{[2
]}

Regev, Aviv ^{[4
]}

Nielsen, Finn Cilius ^{[5
]}

Dor, Yuval ^{[3
]}

Eden, Amir ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Silberman Inst Life Sci, Dept Cell & Dev Biol, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Silberman Inst Life Sci, Dept Genet, IL-91904 Jerusalem, Israel

[3] Hebrew Univ Jerusalem, Dept Dev Biol & Canc Res, Inst Med Res Israel Canada, Hadassah Med Sch, IL-91120 Jerusalem, Israel

[4] Broad Inst MIT & Harvard, Cambridge Ctr 7, Cambridge, MA 02142 USA

[5] Univ Copenhagen, Rigshosp, Ctr Genom Med, DK-2100 Copenhagen, Denmark

[6] Univ Ghent VIB, Dept Plant Syst Biol, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium

来源：

DEVELOPMENTAL CELL | 2012年 / 23卷 / 04期

基金：

以色列科学基金会;

关键词：

HEPATIC PROGENITOR CELLS; HEPATOCELLULAR-CARCINOMA; EXPRESSION PROFILES; CYCLE PROGRESSION; GENE-EXPRESSION; BETA-CELL; REGENERATION; DESTRUCTION; LOCALIZATION; HEPATOCYTES;

D O I：

10.1016/j.devcel.2012.08.009

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Most adult mammalian tissues are quiescent, with rare cell divisions serving to maintain homeostasis. At present, the isolation and study of replicating cells from their in vivo niche typically involves immunostaining for intracellular markers of proliferation, causing the loss of sensitive biological material. We describe a transgenic mouse strain, expressing a CyclinB1-GFP fusion reporter, that marks replicating cells in the S/G2/M phases of the cell cycle. Using flow cytometry, we isolate live replicating cells from the liver and compare their transcriptome to that of quiescent cells to reveal gene expression programs associated with cell proliferation in vivo. We find that replicating hepatocytes have reduced expression of genes characteristic of liver differentiation. This reporter system provides a powerful platform for gene expression and metabolic and functional studies of replicating cells in their in vivo niche.

引用

页码：681 / 690

页数：10

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