Fluorescence imaging lysosomal changes during cell division and apoptosis observed using Nile Blue based near-infrared emission

被引：48

作者：

Fan, Jiangli ^{[1
]}

Dong, Huijuan ^{[1
]}

Hu, Mingming ^{[1
]}

Wang, Jingyun ^{[2
]}

Zhang, Hua ^{[1
]}

Zhu, Hao ^{[1
]}

Sun, Wen ^{[1
]}

Peng, Xiaojun ^{[1
]}

机构：

[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

[2] Dalian Univ Technol, Dept Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 07期

关键词：

CYSTEINE CATHEPSINS; TRAFFICKING; DYNAMICS; PROBES; ROLES;

D O I：

10.1039/c3cc48043g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

NBM, a Nile Blue based NIR fluorescent dye was reported. NBM is highly effective in specifically labeling lysosomes with low concentration and good photostability in various kinds of live cells. It was first successfully used to observe the disappearance and reproduction of lysosomes during cell division.

引用

页码：882 / 884

页数：3

共 28 条

[1] A VIEW OF ACIDIC INTRACELLULAR COMPARTMENTS [J].

ANDERSON, RGW ;

ORCI, L .

JOURNAL OF CELL BIOLOGY, 1988, 106 (03) :539-543

[2] MOLECULAR SIZE-FRACTIONATION DURING ENDOCYTOSIS IN MACROPHAGES [J].

BERTHIAUME, EP ;

MEDINA, C ;

SWANSON, JA .

JOURNAL OF CELL BIOLOGY, 1995, 129 (04) :989-998

[3] A long-wavelength fluorescent chemodosimeter selective for Cu(II) ion in water [J].

Dujols, V ;

Ford, F ;

Czarnik, AW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (31) :7386-7387

[4] Nuclear membrane dynamics and reassembly in living cells: Targeting of an inner nuclear membrane protein in interphase and mitosis [J].

Ellenberg, J ;

Siggia, ED ;

Moreira, JE ;

Smith, CL ;

Presley, JF ;

Worman, HJ ;

LippincottSchwartz, J .

JOURNAL OF CELL BIOLOGY, 1997, 138 (06) :1193-1206

[5] AN ULTRASTRUCTURAL-STUDY OF MITOSIS AND CYTOKINESIS IN NORMAL RESTING HUMAN-BREAST [J].

FERGUSON, DJP .

CELL AND TISSUE RESEARCH, 1988, 252 (03) :581-587

[6] Two-Step Assembly Dynamics of the Bacillus subtilis Divisome [J].

Gamba, Pamela ;

Veening, Jan-Willem ;

Saunders, Nigel J. ;

Hamoen, Leendert W. ;

Daniel, Richard A. .

JOURNAL OF BACTERIOLOGY, 2009, 191 (13) :4186-4194

[7] Fluorescent Indicators for Intracellular pH [J].

Han, Junyan ;

Burgess, Kevin .

CHEMICAL REVIEWS, 2010, 110 (05) :2709-2728

[8] Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker [J].

Hanaki, K ;

Momo, A ;

Oku, T ;

Komoto, A ;

Maenosono, S ;

Yamaguchi, Y ;

Yamamoto, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 302 (03) :496-501

[9]

Haugland R. P, 2005, GUIDE FLUORESCENT PR

[10] Subcellular trafficking of antisense oligonucleotides and down-regulation of bcl-2 gene expression in human melanoma cells using a fusogenic liposome delivery system [J].

Hu, Q ;

Bally, MB ;

Madden, TD .

NUCLEIC ACIDS RESEARCH, 2002, 30 (16) :3632-3641

← 1 2 3 →