Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots

被引：165
作者：
Fuzzati, N [1 ]
Gabetta, B [1 ]
Jayakar, K [1 ]
Pace, R [1 ]
Peterlongo, F [1 ]
机构：
[1] Indena SPA, Lab Ric & Sviluppo, I-20090 Milan, Italy
来源：
JOURNAL OF CHROMATOGRAPHY A | 1999年 / 854卷 / 1-2期
关键词：
Panax ginseng; plant materials; ginsenosides;
D O I：
10.1016/S0021-9673(99)00463-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatographic method was developed for electrospray mass spectrometric analysis of ginsenosides in Panax ginseng roots. The analyses were performed on a reversed-phase C-18 column using a binary eluent (aqueous 8 mM NH4OAc, buffered to pH 7 with NH4OH-acetonitrile) under gradient conditions. Twenty-five ginsenosides could be separated and detected. The mass spectra obtained provided information on their molecular masses. A MS-MS experiment was undertaken in order to determine the sugar unit sequences and the aglycone moieties. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：69 / 79
页数：11
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