Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots

被引：165

作者：

Fuzzati, N ^{[1
]}

Gabetta, B ^{[1
]}

Jayakar, K ^{[1
]}

Pace, R ^{[1
]}

Peterlongo, F ^{[1
]}

机构：

[1] Indena SPA, Lab Ric & Sviluppo, I-20090 Milan, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 854卷 / 1-2期

关键词：

Panax ginseng; plant materials; ginsenosides;

D O I：

10.1016/S0021-9673(99)00463-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic method was developed for electrospray mass spectrometric analysis of ginsenosides in Panax ginseng roots. The analyses were performed on a reversed-phase C-18 column using a binary eluent (aqueous 8 mM NH4OAc, buffered to pH 7 with NH4OH-acetonitrile) under gradient conditions. Twenty-five ginsenosides could be separated and detected. The mass spectra obtained provided information on their molecular masses. A MS-MS experiment was undertaken in order to determine the sugar unit sequences and the aglycone moieties. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：69 / 79

页数：11

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