Three-dimensional visualization of mixed species biofilm formation together with its substratum

被引：24

作者：

Inaba, Tomohiro ^{[1
]}

Ichihara, Takahiro ^{[1
]}

Yawata, Yutaka ^{[2
]}

Toyofuku, Masanori ^{[1
]}

Uchiyama, Hiroo ^{[1
]}

Nomura, Nobuhiko ^{[1
]}

机构：

[1] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan

[2] MIT, Civil & Environm Engn Parsons Lab, Cambridge, MA 02139 USA

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2013年 / 57卷 / 08期

基金：

日本科学技术振兴机构;

关键词：

Microscopy; oral biofilm; three dimensional visualization; MICROFLUIDIC DEVICE; DENTAL PLAQUE; ARCHITECTURE; EXOPOLYSACCHARIDE; EXPRESSION;

D O I：

10.1111/1348-0421.12064

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Biofilms, such as dental plaque, are aggregates of microorganisms attached to a surface. Thus, visualization of biofilms together with their attached substrata is important in order to understand details of the interaction between them. However, so far there is limited availability of such techniques. Here, non-invasive visualization of biofilm formation with its attached substratum by applying the previously reported technique of continuous-optimizing confocal reflection microscopy (COCRM) is reported. The process of development of oral biofilm together with its substratum was sequentially visualized with COCRM. This study describes a convenient method for visualizing biofilm and its attached surface.

引用

页码：589 / 593

页数：5

共 20 条

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