Crustacean hyperglycemic hormone of Portunus trituberculatus: evidence of alternative splicing and potential roles in osmoregulation

被引：19
作者：
Sun, Dongfang [1 ]
Lv, Jianjian [1 ,2 ]
Gao, Baoquan [1 ,2 ]
Liu, Ping [1 ,2 ]
Li, Jian [1 ,2 ]
机构：
[1] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr PR China, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Shandong, Peoples R China
[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, 1 Wenhai Rd, Qingdao, Shandong, Peoples R China
来源：
CELL STRESS & CHAPERONES | 2019年 / 24卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Portunus trituberculatus; Crustacean hyperglycemic hormone; Alternative splicing; Osmoregulation; BILATERAL EYESTALK ABLATION; ION-TRANSPORT; CARBONIC-ANHYDRASE; PENAEUS-MONODON; ATPASE ACTIVITY; CRAB; EXPRESSION; GENE; GILL; CLONING;
D O I：
10.1007/s12192-019-00980-6
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The crustacean hyperglycemic hormone (CHH) gene of Portunus trituberculatus (Pt-CHH) consists of four exons and three introns spanning 3849bp in size and generating two mature mRNA, Pt-CHH1, and Pt-CHH2. The primary gene transcript produces a cDNA encoding for the putative Pt-CHH2 from exons 1, 2, 3, and 4 and an alternative transcript encodes for a putative Pt-CHH1 peptide from exons 1, 2, and 4. A promoter fragment of about 3kb was obtained by genomic walking. The tissue-specific expression pattern is examined by reverse transcriptase chain reaction, and the results show that Pt-CHH1 is detected in the eyestalk, brain, muscle, and blood. However, Pt-CHH2 is detected in the ganglia thoracalis and gill. The results indicate that the expression of Pt-CHH2 in the gill might suggest a potential role in osmoregulation. The Pt-CHH transcript level in the gill increases when the crab is exposed to low salinity. The injection of dsRNA for Pt-CHH causes a significant reduction in Pt-CHH2 transcript level and the activity of Na+/K+-ATPase, and carbonic anhydrase (CA) show a serious decrease. In conclusion, this study provides molecular evidence to support the osmoregulatory function of Pt-CHH2.
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收藏
页码：517 / 525
页数：9
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