Copper nanoclusters as peroxidase mimetics and their applications to H2O2 and glucose detection

被引：333

作者：

Hu, Lianzhe ^{[1
,2
]}

Yuan, Yali ^{[1
,2
]}

Zhang, Ling ^{[1
,2
]}

Zhao, Jianming ^{[1
,2
]}

Majeed, Saadat ^{[1
,2
,3
]}

Xu, Guobao ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Grad Univ, Beijing 100864, Peoples R China

[3] Bahauddin Zakaryia Univ, Dept Chem, Multan 60800, Pakistan

来源：

ANALYTICA CHIMICA ACTA | 2013年 / 762卷

基金：

中国国家自然科学基金;

关键词：

Enzyme mimics; Nanoclusters; Biosensors; Copper; Peroxidase; FLUORESCENT DETECTION; GOLD NANOPARTICLES; CLUSTERS; SENSOR; ION;

D O I：

10.1016/j.aca.2012.11.056

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Copper nanoclusters (Cu NCs) are found to possess intrinsic peroxidase-like activity for the first time. Similar to nature peroxidase, they can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by H2O2 to produce a nice blue color reaction. Compared with horseradish peroxidase, Cu NCs exhibits higher activity near neutral pH, which is beneficial for biological applications. The increase in absorbance caused by the Cu NCs catalytic reaction allows the detection of H2O2 in the range of 10 mu M to 1 mM with a detection limit of 10 mu M. A calorimetric method for glucose detection was also developed by combining the Cu NCs catalytic reaction and the enzymatic oxidation of glucose with glucose oxidase. Taking into account the advantages of ultra-small size, good stability, and high biocompatibility in aqueous solutions, Cu NCs are expected to have potential applications in biotechnology and clinical diagnosis as enzymatic mimics. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：83 / 86

页数：4

共 22 条

[1] Fluorescent silver nanoclusters [J].