Bio-macromolecular dynamic structures and functions, illustrated with DNA, antibody, and lipoprotein

被引：2

作者：

Gou, Lu ^{[1
]}

Jin, Taoli ^{[1
]}

Chen, Shuyu ^{[1
]}

Li, Na ^{[1
]}

Hao, Dongxiao ^{[1
]}

Zhang, Shengli ^{[1
]}

Zhang, Lei ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Dept Appl Phys, Sch Sci, Xian 710049, Shaanxi, Peoples R China

来源：

CHINESE PHYSICS B | 2018年 / 27卷 / 02期

基金：

中国国家自然科学基金;

关键词：

bio-macromolecule; single-molecule method; cryo-electron microscopy; electron tomography; CRYO-EM STRUCTURE; NEGATIVE-STAINING PROTOCOL; MAGNETIC TWEEZERS; CRYOELECTRON MICROSCOPY; ELECTRON TOMOGRAPHY; OPTICAL TWEEZERS; SINGLE; PROTEIN; SPECTROSCOPY; RESOLUTION;

D O I：

10.1088/1674-1056/27/2/028708

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Bio-macromolecules, such as proteins and nucleic acids, are the basic materials that perform fundamental activities required for life. Their structural heterogeneities and dynamic personalities are vital to understand the underlying functional mechanisms of bio-macromolecules. With the rapid development of advanced technologies such as single-molecule technologies and cryo-electron microscopy (cryo-EM), an increasing number of their structural details and mechanics properties at molecular level have significantly raised awareness of basic life processes. In this review, firstly the basic principles of single-molecule method and cryo-EM are summarized, to shine a light on the development in these fields. Secondly, recent progress driven by the above two methods are underway to explore the dynamic structures and functions of DNA, antibody, and lipoprotein. Finally, an outlook is provided for the further research on both the dynamic structures and functions of bio-macromolecules, through single-molecule method and cryo-EM combining with molecular dynamics simulations.

引用

页数：8

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