A 3D Printable Electroconductive Biocomposite Bioink Based on Silk Fibroin-Conjugated Graphene Oxide

被引：59

作者：

Ajiteru, Olatunji ^{[1
]}

Sultan, Tipu ^{[1
]}

Lee, Young Jin ^{[1
]}

Seo, Ye Been ^{[1
]}

Hong, Heesun ^{[1
]}

Lee, Ji Seung ^{[1
]}

Lee, Hanna ^{[1
]}

Suh, Ye Ji ^{[1
]}

Ju, Hyung Woo ^{[2
]}

Lee, Ok Joo ^{[1
]}

Park, Hae Sang ^{[1
,3
]}

Jang, Moongyu ^{[4
,5
]}

Kim, Soon Hee ^{[1
]}

Park, Chan Hum ^{[1
,3
]}

机构：

[1] Hallym Univ, Nanobio Regenerat Med Inst, Chunchon 24252, Gangwon Do, South Korea

[2] Nanobio Regenerat Technol Co Ltd 56 16, Chunchon 24427, Gangwon Do, South Korea

[3] Hallym Univ, Chuncheon Sacred Heart Hosp, Sch Med, Dept Otorhinolaryngol Head & Neck Surg, Chunchon 24252, Gangwon Do, South Korea

[4] Hallym Univ, Sch Nano Convergence Technol, Chunchon 24252, Gangwon Do, South Korea

[5] Hallym Univ, Nano Convergence Technol Res Ctr, Chunchon 24252, Gangwon Do, South Korea

来源：

NANO LETTERS | 2020年 / 20卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

Graphene oxide; silk fibroin; electrical conductivity; neurogenesis; STEM-CELL DIFFERENTIATION; ELECTRICAL-STIMULATION; MECHANICAL-PROPERTIES; SCAFFOLDS; FABRICATION; NANOSHEETS; MEMBRANE; ADHESION;

D O I：

10.1021/acs.nanolett.0c02986

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reduced graphene oxide (rGO) has wide application as a nanofiller in the fabrication of electroconductive biocomposites due to its exceptional properties. However, the hydrophobicity and chemical stability of rGO limit its ability to be incorporated into precursor polymers for physical mixing during biocomposite fabrication. Moreover, until now, no suitable rGO-combining biomaterials that are stable, soluble, biocompatible, and 3D printable have been developed. In this study, we fabricated digital light processing (DLP) printable bioink (SGOB1), through covalent reduction of graphene oxide (GO) by glycidyl methacrylated silk fibroin (SB). Compositional analyses showed that SGOB1 contains approximately 8.42% GO in its reduced state. Our results also showed that the rGO content of SGOB1 became more thermally stable and highly soluble. SGOB1 hydrogels demonstrated superior mechanical, electroconductive, and neurogenic properties than (SB). Furthermore, the photocurable bioink supported Neuro2a cell proliferation and viability. Therefore, SGOB1 could be a suitable biocomposite for neural tissue engineering.

引用

页码：6873 / 6883

页数：11

共 49 条

[1] Rolled graphene oxide foams as three-dimensional scaffolds for growth of neural fibers using electrical stimulation of stem cells [J].