Influence of the Immune Microenvironment Provided by Implanted Biomaterials on the Biological Properties of Masquelet-Induced Membranes in Rats: Metakaolin as an Alternative Spacer

被引:4
作者
Durand, Marjorie [1 ]
Oger, Myriam [2 ]
Nikovics, Krisztina [2 ]
Venant, Julien [1 ,3 ]
Guillope, Anne-Cecile [1 ]
Jouve, Eugenie [1 ]
Barbier, Laure [4 ]
Begot, Laurent [2 ]
Poirier, Florence [3 ]
Rousseau, Catherine [4 ]
Pitois, Olivier [5 ]
Mathieu, Laurent [1 ,6 ]
Favier, Anne-Laure [2 ]
Lutomski, Didier [3 ]
Collombet, Jean-Marc [1 ]
机构
[1] French Armed Forces Biomed Res Inst, Dept Med & Surg Assistance Armed Forces, OsteoArticulary Biotherapy Unit, F-91223 Bretigny Sur Orge, France
[2] French Armed Forces Biomed Res Inst, Dept Platforms & Technol Res, Imaging Unit, F-91223 Bretigny Sur Orge, France
[3] Sorbonne Paris Nord Univ, Tissue Engn Res Unit URIT, F-93000 Bobigny, France
[4] French Armed Forces Biomed Res Inst, Dept Platforms & Technol Res, Mol Biol Unit, F-91223 Bretigny Sur Orge, France
[5] Gustave Eiffel Univ, Lab Navier, CNRS, Ecole Ponts ParisTech, F-77447 Marne La Vallee, France
[6] French Mil Hlth Serv Acad, Dept Surg, Ecole Val Grace, 1 Pl Alphonse Laveran, F-75005 Paris, France
关键词
Masquelet-induced membrane; macrophages; PMMA; metakaolin; MACROPHAGE PHENOTYPE; BONE; CELLS; FRACTURE; EXPRESSION; SCAFFOLDS; PCR;
D O I
10.3390/biomedicines10123017
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Macrophages play a key role in the inflammatory phase of wound repair and foreign body reactions-two important processes in the Masquelet-induced membrane technique for extremity reconstruction. The macrophage response depends largely on the nature of the biomaterials implanted. However, little is known about the influence of the macrophage microenvironment on the osteogenic properties of the induced membrane or subsequent bone regeneration. We used metakaolin, an immunogenic material, as an alternative spacer to standard polymethylmethacrylate (PMMA) in a Masquelet model in rats. Four weeks after implantation, the PMMA- and metakaolin-induced membranes were harvested, and their osteogenic properties and macrophage microenvironments were investigated by histology, immunohistochemistry, mass spectroscopy and gene expression analysis. The metakaolin spacer induced membranes with higher levels of two potent pro-osteogenic factors, transforming growth factor-beta (TGF-beta) and bone morphogenic protein-2 (BMP-2). These alternative membranes thus had greater osteogenic activity, which was accompanied by a significant expansion of the total macrophage population, including both the M1-like and M2-like subtypes. Microcomputed tomographic analysis showed that metakaolin-induced membranes supported bone regeneration more effectively than PMMA-induced membranes through better callus properties (+58%), although this difference was not significant. This study provides the first evidence of the influence of the immune microenvironment on the osteogenic properties of the induced membranes.
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页数:17
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