Direct sequencing of the white spot syndrome virus from Brazil: Genome assembly and new insights on phylogeny

被引:11
作者
Dantas, Marcia Danielle A. [1 ,2 ]
Teixeira, Diego G. [2 ]
Silva-Portela, Rita Cassia B. [4 ]
Soares, Paulo Eduardo T. [1 ,3 ]
Lima, Joao Paulo M. S. [2 ,3 ]
Agnez-Lima, Lucymara F. [2 ,3 ,4 ]
Lanza, Daniel Carlos F. [1 ,2 ,3 ]
机构
[1] Univ Fed Rio Grande do Norte, Dept Biochem, Appl Mol Biol Lab LAPLIC, Natal, RN, Brazil
[2] Univ Fed Rio Grande do Norte, Postgrad Program Biochem, Natal, RN, Brazil
[3] Univ Fed Rio Grande do Norte, Postgrad Program Bioinformat, Natal, RN, Brazil
[4] Univ Fed Rio Grande do Norte, Dept Cellular Biol & Genet, Lab Mol Biol & Genom, Natal, RN, Brazil
关键词
WSSV; Shrimp farming; Homologous regions; Recombination; NUCLEAR POLYHEDROSIS-VIRUS; FUNCTIONAL-ANALYSIS; HISTORIC EMERGENCE; SHRIMP PATHOGENS; ALIGNMENT; VIRULENCE; IDENTIFICATION; STRAINS; ORIGINS; REGIONS;
D O I
10.1016/j.virusres.2017.12.006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
White spot syndrome virus (WSSV) has been the cause of great economic losses in world shrimp farming. In this work the genome of a Brazilian WSSV isolate was determined from direct sequencing of total DNA extracted from an infected whiteleg shrimp, and assembled based on a chimera template approach. Comparisons between WSSV-BR and other isolates revealed that the Brazilian virus has a relatively small genome, and is very similar to isolates from Thailand and Mexico. A phylogenetic relationship using different approaches has demonstrated that these isolates share a common evolutionary history. An analysis of conflicting phylogenetic signals also considering genomes of other isolates revealed that the evolutionary history of WSSV may be related to recombination events. We observed that these events can also be traced at some level by analyzing the homologous regions in the WSSV genome. The existence of recombination events introduces a new point of view that must be considered in the evolutionary history of WSSV.
引用
收藏
页码:52 / 61
页数:10
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