Selective detection of cysteine over homocysteine and glutathione by a bis(bromoacetyl)fluorescein probe

被引：46

作者：

Hong, Keum-Hee ^{[1
]}

Lim, Soo-Yeon ^{[1
]}

Yun, Mi-Yeon ^{[2
]}

Lim, Joo-Won ^{[3
]}

Woo, Je-Hyuk ^{[3
]}

Kwon, Hyockman ^{[2
]}

Kim, Hae-Jo ^{[1
]}

机构：

[1] Hankuk Univ Foreign Studies, Dept Chem, Yongin 449791, South Korea

[2] Hankuk Univ Foreign Studies, Dept Biosci & Biotechnol, Yongin 449791, South Korea

[3] Korea Adv Inst Sci & Technol, Korea Sci Acad, Pusan 614822, South Korea

来源：

TETRAHEDRON LETTERS | 2013年 / 54卷 / 23期

基金：

新加坡国家研究基金会;

关键词：

Chemodosimeter; Cysteine; Fluorescence; Probe; TURN-ON PROBE; FLUORESCENCE; SENSOR;

D O I：

10.1016/j.tetlet.2013.03.119

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A bromoacetyl functionalized fluorescein chemodosimeter (1) was utilized as a fluorescent probe for cysteine. The probe showed a selective and sensitive response to cysteine over homocysteine and glutathione in aqueous buffer through a rapid cyclization reaction. When cysteine was added, a fast fluorescence turn-on change of 1 was observed, thus allowing cysteine to be discriminated from other biothiols and micromolar concentrations of cysteine to be detected by the naked eye. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：3003 / 3006

页数：4

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