An approach for extraction of kernel oil from Pinus pumila using homogenate-circulating ultrasound in combination with an aqueous enzymatic process and evaluation of its antioxidant activity

被引:60
作者
Chen, Fengli [1 ]
Zhang, Qiang [2 ]
Gu, Huiyan [2 ]
Yang, Lei [1 ]
机构
[1] Northeast Forestry Univ, Minist Educ, Key Lab Forest Plant Ecol, Harbin 150040, Peoples R China
[2] Northeast Forestry Univ, Sch Forestry, Harbin 150040, Peoples R China
基金
中国国家自然科学基金;
关键词
Homogenate-circulating ultrasound in combination with aqueous enzymatic extraction; Pinus pumila; Kernel oil; Delta 5-unsaturated polymethylene interrupted fatty acids; XANTHOCERAS-SORBIFOLIA BUNGE; CARBON-DIOXIDE EXTRACTION; ASSISTED EXTRACTION; LIQUID-CHROMATOGRAPHY; SOLVENT-EXTRACTION; PHENOLIC-COMPOUNDS; SEED KERNELS; NUT OIL; ACID; CRYSTALLIZATION;
D O I
10.1016/j.chroma.2016.10.037
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In this study, a novel approach involving homogenate-circulating ultrasound in combination with aqueous enzymatic extraction (H-CUAEE) was developed for extraction of kernel oil from Pinus pumila. Following comparison of enzyme types and concentrations, an enzyme mixture consisting of cellulase, pectinase and hemicellulase (1:1:1, w/w/w) at a concentration of 2.5% was selected and applied for effective oil extraction and release. Several variables potentially influencing extraction yields, namely, homogenization time, incubation temperature, incubation time, mark-space ratio of ultrasound irradiation, ultrasound irradiation power, liquid-solid ratio, pH and stirring rate, were optimized by Plackett-Burman design. Among the eight variables, incubation temperature, incubation time and liquid solid ratio were statistically significant and were further optimized by Box-Behnken design to predict optimum extraction conditions and ascertain operability ranges for maximum extraction yield. Under optimum operating conditions, extraction yields of P. pumila kernel oil were 31.89 +/- 11.12% with a Delta 5-unsaturated polymethylene interrupted fatty acid content of 20.07% and an unsaturated fatty acid content of 93.47%. Our study results indicate that the proposed H-CUAEE process has enormous potential for efficient and environmentally friendly extraction of edible oils. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:68 / 79
页数:12
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