Global gene expression and functional network analysis of gastric cancer identify extended pathway maps and GPRC5A as a potential biomarker

被引：49
作者：
Cheng, Lei [1 ,2 ,3 ]
Yang, Sheng [2 ,3 ,5 ]
Yang, Yanqing [2 ,3 ]
Zhang, Wen [1 ]
Xiao, Huasheng [1 ]
Gao, Hengjun [1 ,6 ]
Deng, Xiaxing [4 ]
Zhang, Qinghua [2 ,3 ,7 ]
机构：
[1] Natl Engn Ctr Biochip Shanghai, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, State Key Lab Med Genom, Shanghai, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Inst Hematol, Shanghai, Peoples R China
[4] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Gen Surg, Shanghai, Peoples R China
[5] Lab Shanghai Skin Dis Hosp, Shanghai, Peoples R China
[6] Tongji Univ, Tongji Hosp, Inst Digest Dis, Shanghai 200092, Peoples R China
[7] Wayen Biotechnol Inc, Shanghai, Peoples R China
来源：
CANCER LETTERS | 2012年 / 326卷 / 01期
关键词：
Gastric cancer; Expression microarray; Network analysis; Pathway; Biomarker; STEM-CELL ANTIGEN; SHOCK-PROTEIN; 47; COPY NUMBER; IDENTIFICATION; TARGET; CARCINOMA; SURVIVAL; BREAST; PSCA; OVEREXPRESSION;
D O I：
10.1016/j.canlet.2012.07.031
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
To get more understanding of the molecular mechanisms underlying gastric cancer, 25 paired samples were applied to gene expression microarray analysis. Here, expression microarray, quantitative reverse transcription-PCR (qRT-PCR) and immunohistochemical analysis indicated that GPRC5A was significantly elevated in gastric cancer tissues. The integrative network analysis of deregulated genes generated eight subnetworks. We also mapped copy number variations (CNVs) and associated mRNA expression changes into pathways and identified WNT, RTK-Ras-PI3K-AKT, NF-kappa B, and PLAU-JAK-STAT pathways involved in proliferation, evading apoptosis and sustained angiogenesis, respectively. Taken together, our results reveal several interesting genes including GPRC5A as potential biomarkers for gastric cancer, and highlight more systematical insight of deregulated genes in genetic pathways of gastric carcinogenesis. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：105 / 113
页数：9
相关论文
共 54 条
[31]   Overexpression of VCC-1 gene in human hepatocellular carcinoma cells promotes cell proliferation and invasion [J].
Mu, Xia ;
Chen, Yao ;
Wang, Shuihai ;
Huang, Xiang ;
Pan, Huazhen ;
Li, Ming .
ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2009, 41 (08) :631-637
[32]   Integrated gene copy number and expression microarray analysis of gastric cancer highlights potential target genes [J].
Myllykangas, Samuel ;
Junnila, Siina ;
Kokkola, Arto ;
Autio, Reija ;
Scheinin, Ilari ;
Kiviluoto, Tuuia ;
Karjalainen-Lindsberg, Marja-Liisa ;
Hollmen, Jaakko ;
Knuutila, Sakari ;
Puolakkainen, Pauli ;
Monn, Outi .
INTERNATIONAL JOURNAL OF CANCER, 2008, 123 (04) :817-825
[33]   Identification of RAI3 as a therapeutic target for breast cancer [J].
Nagahata, T ;
Sato, T ;
Tomura, A ;
Onda, M ;
Nishikawa, K ;
Emi, M .
ENDOCRINE-RELATED CANCER, 2005, 12 (01) :65-73
[34]   MYC oncogenes and human neoplastic disease [J].
Nesbit, CE ;
Tersak, JM ;
Prochownik, EV .
ONCOGENE, 1999, 18 (19) :3004-3016
[35]  
Nese N, 2010, ANAL QUANT CYTOL, V32, P90
[36]   Oncogenic Pathway Combinations Predict Clinical Prognosis in Gastric Cancer [J].
Ooi, Chia Huey ;
Ivanova, Tatiana ;
Wu, Jeanie ;
Lee, Minghui ;
Tan, Iain Beehuat ;
Tao, Jiong ;
Ward, Lindsay ;
Koo, Jun Hao ;
Gopalakrishnan, Veena ;
Zhu, Yansong ;
Cheng, Lai Ling ;
Lee, Julian ;
Rha, Sun Young ;
Chung, Hyun Cheol ;
Ganesan, Kumaresan ;
So, Jimmy ;
Soo, Khee Chee ;
Lim, Dennis ;
Chan, Weng Hoong ;
Wong, Wai Keong ;
Bowtell, David ;
Yeoh, Khay Guan ;
Grabsch, Heike ;
Boussioutas, Alex ;
Tan, Patrick .
PLOS GENETICS, 2009, 5 (10)
[37]  
Park JY, 2007, ONCOL REP, V18, P377
[38]   Genetic variations of prostate stem cell antigen (PSCA) contribute to the risk of gastric cancer for Eastern Asians: A meta-analysis based on 16792 individuals [J].
Qiao, Lei ;
Feng, Yong .
GENE, 2012, 493 (01) :83-91
[39]   Distribution and expression pattern of claudins 6, 7, and 9 in diffuse- and intestinal-type gastric adenocarcinomas [J].
Rendón-Huerta E. ;
Teresa F. ;
Teresa G.M. ;
Xochitl G.-S. ;
Georgina A.-F. ;
Veronica Z.-Z. ;
Montaño L.F. .
Journal of Gastrointestinal Cancer, 2010, 41 (1) :52-59
[40]   TM4 microarray software suite [J].
Saeed, Alexander I. ;
Hagabati, Nirmal K. ;
Braisted, John C. ;
Liang, Wei ;
Sharov, Vasily ;
Howe, Eleanor A. ;
Li, Jianwei ;
Thiagarajan, Mathangi ;
White, Joseph A. ;
Quackenbush, John .
DNA MICROARRAYS, PART B: DATABASES AND STATISTICS, 2006, 411 :134-+
123456