Clarification of the factors involved in the formation of unusual ancient organic carbon-rich deposits (like eastern Mediterranean sapropels) is central in understanding oceanic carbon cycling. The role of oxygenation remains a subject of controversy primarily due to two major uncertainties: (1) it is unknown if ancient organic-rich deposits reflect an accumulation of refractory organic matter (OM) or oxygenation-related aberrant sediment OM recycling, and (2) although marine OM degradation may be slower under anoxic conditions, its ultimate impact on organic carbon (C-org) preservation over geological time remains unclear. Here we provide direct experimental evidence that the Corg in eastern Mediterranean S1 sapropells (deposited > 5 ka) is still highly reactive and that a shutdown in labile organic matter degradation under anoxic conditions played a key role in the formation of these deposits.