Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system

被引：21

作者：

Inoue, I ^{[1
]}

Wakamoto, Y ^{[1
]}

Yasuda, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Meguro Ku, Tokyo 1538902, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 2001年 / 77卷 / 08期

关键词：

on-chip culture system; single-cell culture; Escherichia coli; cell division time; cell growth speed; competition of sister cells; non-genetic variability;

D O I：

10.2183/pjab.77.145

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To investigate the non-gene tic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in pm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 mum by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.

引用

页码：145 / 150

页数：6

共 9 条

[1] SYNCHRONOUS GROWTH OF CELLS AND GENERATION TIME DISTRIBUTION [J].