Label-Free Multiphoton Microscopy: The Origin of Fluorophores and Capabilities for Analyzing Biochemical Processes

被引：23

作者：

Shirshin, E. A. ^{[1
,2
]}

Yakimov, B. P. ^{[1
]}

Darvin, M. E. ^{[3
,4
,5
,6
]}

Omelyanenko, N. P. ^{[7
]}

Rodionov, S. A. ^{[7
]}

Gurfinkel, Y. I. ^{[8
]}

Lademann, J. ^{[3
,4
,5
,6
]}

Fadeev, V. V. ^{[1
]}

Priezzhev, A. V. ^{[1
]}

机构：

[1] Lomonosov Moscow State Univ, Fac Phys, Moscow 119991, Russia

[2] Russian Acad Sci, Inst Spect, Moscow 108840, Russia

[3] Charite Univ Med Berlin, Dept Dermatol Venerol & Allergol, Ctr Expt & Appl Cutaneous Physiol, Charitepl 1, D-10117 Berlin, Germany

[4] Free Univ Berlin, Charitepl 1, D-10117 Berlin, Germany

[5] Humboldt Univ, Charitepl 1, D-10117 Berlin, Germany

[6] Berlin Inst Hlth, Charitepl 1, D-10117 Berlin, Germany

[7] NN Priorov Natl Med Res Ctr Traumatol & Orthopaed, Moscow 127299, Russia

[8] Lomonosov Moscow State Univ, Med Sci Educ Ctr, Moscow 119192, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2019年 / 84卷 / Suppl 1期

基金：

俄罗斯科学基金会;

关键词：

molecular imaging; multiphoton microscopy; FLIM; autofluorescence; optical harmonic generation; fluorophores; VIVO FLUORESCENCE SPECTROSCOPY; IN-VIVO; HUMAN SKIN; OXIDATIVE STRESS; 2-PHOTON MICROSCOPY; INTRACELLULAR NADH; LASER TOMOGRAPHY; METABOLIC STATE; PHASOR APPROACH; LIFETIME;

D O I：

10.1134/S0006297919140050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Multiphoton microscopy (MPM) is a method of molecular imaging and specifically of intravital imaging that is characterized by high spatial resolution in combination with a greater depth of penetration into the tissue. MPM is a multimodal method based on detection of nonlinear optical signals-multiphoton fluorescence and optical harmonics-and also allows imaging with the use of the parameters of fluorescence decay kinetics. This review describes and discusses photophysical processes within major reporter molecules used in MPM with endogenous contrasts and summarizes several modern experiments that illustrate the capabilities of label-free MPM for molecular imaging of biochemical processes in connective tissue and cells.

引用

页码：69 / 88

页数：20

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