Three-Dimensional Ultralarge-Pore Ia3d Mesoporous Silica with Various Pore Diameters and Their Application in Biomolecule Immobilization

被引：95

作者：

Vinu, Ajayan ^{[1
]}

Gokulakrishnan, Narasimhan ^{[1
]}

Balasubramanian, Veerappan V. ^{[1
]}

Alam, Sher ^{[1
]}

Kapoor, Mahendra P. ^{[2
]}

Ariga, Katsuhiko ^{[1
]}

Mori, Toshiyuki ^{[3
]}

机构：

[1] Natl Inst Mat Sci, Int Ctr Mat Nanoarchitecton, WPI Res Ctr, Tsukuba, Ibaraki 3050044, Japan

[2] Taiyo Kagaku Co Ltd, Res & Dev Interface Solut Div, Yokaichi, Mie 5100844, Japan

[3] Natl Inst Mat Sci, Nanoion Mat Grp, Tsukuba, Ibaraki 3050044, Japan

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2008年 / 14卷 / 36期

关键词：

adsorption; hydrothermal synthesis; immobilization; mesoporous materials; silica;

D O I：

10.1002/chem.200801304

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Highly Ordered mesoporous three-dimensional Ia3d silica (KIT-6) with different pore diameters has been synthesized by using pluronic P123 as surfactant template and n-butanol its cosolvent at different synthesis temperatures in a highly acidic medium. The materials were characterized by XRD and N-2 adsorption. The synthesis temperature plays a significant role in controlling the pore diameter, surface area. and Pore volume of file materials. The material prepared at 150 degrees C KIT-6-150, has a large pore diameter ( 11.3 nm) and it high specific pore volume ( 1.53 cm(3) g(-1)). We also demonstrate immobilization of lysozyme, which is a stable and hard protein, Oil KIT-6 materials with different pore diameters. The amount of lysozyme adsorbed on large-pore KIT-6 is extremely large (57.2 mu mol g(-1)) and is much higher than that observed for mesoporous silicas MCM-41, SBA-15. and KIT-5, mesoporous carbons, and carbon nanocages. The effect of various parameters such its buffer concentration, adsorption temperature, concentration of the lysozyme, and the texturtal parameter of the adsorbent on the lysozyme adsorption capacity of KIT-6 was studied. The amount adsorbed mainly depends oil solution pH, ionic strength. adsorption temperature. and Pore volume and pore diameter of the adsorbent. The mechanism of adsorption on KIT-6 under different adsorption conditions is discussed. In addition, the structural stability of lysozyme molecules and the KIT-6 adsorbent before and after adsorption were investigated by XRD. nitrogen adsorption, and FTIR spectroscopy.

引用

页码：11529 / 11538

页数：10

共 67 条

[1] Surface-mediated folding and misfolding of proteins at lipid/water interfaces [J].

Adams, S ;

Higgins, AM ;

Jones, RAL .

LANGMUIR, 2002, 18 (12) :4854-4861

[2]

ANDRADE JD, 1986, ADV POLYM SCI, V79, P1

[3]

ANDRADE JD, 1985, SURFACE INTERFACIAL, V2

[4] THE BEHAVIOR OF SOME MODEL PROTEINS AT SOLID LIQUID INTERFACES .1. ADSORPTION FROM SINGLE PROTEIN SOLUTIONS [J].

ARAI, T ;

NORDE, W .

COLLOIDS AND SURFACES, 1990, 51 :1-15

[5] ENZYMES AND OTHER PROTEINS ENTRAPPED IN SOL-GEL MATERIALS [J].

AVNIR, D ;

BRAUN, S ;

LEV, O ;

OTTOLENGHI, M .

CHEMISTRY OF MATERIALS, 1994, 6 (10) :1605-1614

[6] Analysis of hen egg white lysozyme adsorption on Si(Ti)O2 | aqueous solution interfaces at low ionic strength:: a biphasic reaction related to solution self-association [J].

Ball, V ;

Ramsden, JJ .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2000, 17 (02) :81-94

[7] Lag phases in the adsorption of lysozyme to Si(Ti)O2 surfaces in the presence of sodium thiocyanate.: Part I.: Phenomenology [J].

Ball, V ;

Lustig, A ;

Ramsden, JJ .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 1999, 1 (15) :3667-3671

[8] Absence of surface exclusion in the first stage of lysozyme adsorption is driven through electrostatic self-assembly [J].

Ball, V ;

Ramsden, JJ .

JOURNAL OF PHYSICAL CHEMISTRY B, 1997, 101 (28) :5465-5469

[9] STRUCTURE OF HEN EGG-WHITE LYSOZYME - A 3-DIMENSIONAL FOURIER SYNTHESIS AT 2A RESOLUTION [J].

BLAKE, CCF ;

KOENIG, DF ;

MAIR, GA ;

NORTH, ACT ;

PHILLIPS, DC ;

SARMA, VR .

NATURE, 1965, 206 (4986) :757-&

[10]

Brash J L, 1995, PROTEINS INTERFACES

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