Overproduction of superoxide dismutase and catalase confers cassava resistance to Tetranychus cinnabarinus

被引：54

作者：

Lu, Fuping ^{[1
]}

Liang, Xiao ^{[1
]}

Lu, Hui ^{[1
]}

Li, Qian ^{[1
]}

Chen, Qing ^{[1
]}

Zhang, Peng ^{[2
]}

Li, Kaimian ^{[3
]}

Liu, Guanghua ^{[4
]}

Yan, Wei ^{[4
]}

Song, Jiming ^{[4
]}

Duan, Chunfang ^{[4
]}

Zhang, Linhui ^{[4
]}

机构：

[1] Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant,Natl Key Lab Plant M, Shanghai 200032, Peoples R China

[3] Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China

[4] Yunnan Acad Agr Sci, Inst Trop & Subtrop Cash Crops, Baoshan 678025, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

OXIDATIVE STRESS; REACTIVE OXYGEN; ANTIOXIDATIVE ENZYMES; ENHANCED TOLERANCE; SPIDER-MITE; PLANTS; CHLOROPLASTS; SEEDLINGS; PARAQUAT; GENE;

D O I：

10.1038/srep40179

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To explore the role of protective enzymes in cassava (Manihot esculenta Crantz) resistance to mites, transgenic cassava lines overproducing copper/zinc superoxide dismutase (MeCu/ZnSOD) and catalase (MeCAT1) were used to evaluate and molecularly confirm cassava resistance to Tetranychus cinnabarinus. Laboratory evaluation demonstrated that, compared with the control cultivar TMS60444 (wild type, WT), the survival, reproduction, development and activities of SOD and CAT in T. cinnabarinus feeding on transgenic cassava lines SC2, SC4, and SC11 significantly inhibited. Furthermore, the activities of SOD and CAT in transgenic cassava lines SC2, SC4, and SC11 damaged by T. cinnabarinus significantly increased. These findings were similar to the results in the mite-resistant cassava cultivars. Besides, field evaluation indicated that the transgenic cassava lines SC2, SC4, and SC11 were slightly damaged as the highly mite-resistant control C1115, while the highly mite-susceptible WT was severely damaged by T. cinnabarinus. Laboratory and field evaluation demonstrated that transgenic cassava lines were resistant to T. cinnabarinus, which directly confirmed that the increase in SOD and CAT activities was positively related to cassava resistance to T. cinnabarinus. These results will help in understanding the antioxidant defense responses in the cassava-mite interaction and molecular breeding of mite-resistant cassava for effective pest control.

引用

页数：13

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