EFFECTS OF MATRINE ON JAK-STAT SIGNALING TRANSDUCTION PATHWAYS IN BLEOMYCIN-INDUCED PULMONARY FIBROSIS

被引：57

作者：

Ma, Xiuqin ^{[1
]}

Chen, Ruhua ^{[1
]}

Liu, Xiufang ^{[1
]}

Xie, Jin ^{[1
]}

Si, Keyun ^{[1
]}

Duan, Lirong ^{[2
]}

机构：

[1] Yixing People Hosp, Dept Resp Dis, Yixing 214200, Peoples R China

[2] Jiangsu Univ, Coll Med, Zhenjiang 212001, Peoples R China

来源：

AFRICAN JOURNAL OF TRADITIONAL COMPLEMENTARY AND ALTERNATIVE MEDICINES | 2013年 / 10卷 / 03期

关键词：

Pulmonary fibrosis; Bleomycin; Matrine; Signaling Transduction pathway; EXPRESSION; MICE; PATHOGENESIS; APOPTOSIS; ACTIVATOR; GENE; RAT;

D O I：

10.4314/ajtcam.v10i3.10

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

The current study aims to investigate the effects of matrine on the JAK-STAT signaling transduction pathways in bleomycin (BLM)-induced pulmonary fibrosis (PF) and to explore its action mechanism. A total of 72 male C57BL/6 mice were randomized into the control, model, and treatment groups. PF models were established by instilling BLM intratracheally. The treatment group was given daily matrine through gastric lavage. Six mice were sacrificed in each group at 3, 7, 14, and 28 days. The lung tissues were observed using hematoxylin-eosin staining. The expression of JAK, STAT1, and STAT3 was observed using immunohistochemistry and then determined using real-time polymerase chain reaction. Alveolitis and PF significantly improved in the treatment group compared with the model group (P < 0.05). The expression of JAK, STAT1, and STAT3 in the model group increased at day 7, peaked at day 14 and then decreased, but the expression was still higher than that in the control group at day 28 (P < 0.05). The three indices in the treatment group were significantly lower than those in the model group at any detection time point (P < 0.05). PF causes high expression of JAK, STAT1, and STAT3. Matrine exerts an anti-PF effect by inhibiting the JAK-STAT signaling transduction pathways.

引用

页码：442 / 448

页数：7

共 21 条

[1] STAT6 activation by Toxoplasma gondii infection induces the expression of Th2 C-C chemokine ligands and B clade serine protease inhibitors in macrophage [J].

Ahn, Hye-Jin ;

Kim, Ji Yeon ;

Ryu, Kyung-Ju ;

Nam, Ho-Woo .

PARASITOLOGY RESEARCH, 2009, 105 (05) :1445-1453

[2] Bleomycin-induced pulmonary fibrosis in transgenic mice that either lack or overexpress the murine plasminogen activator inhibitor-1 gene [J].

Eitzman, DT ;

McCoy, RD ;

Zheng, XX ;

Fay, WP ;

Shen, TL ;

Ginsburg, D ;

Simon, RH .

JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (01) :232-237

[3]

Fan Xian-ming, 2003, Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, V19, P3

[4] Physiologically Based Pharmacokinetics of Matrine in the Rat after Oral Administration of Pure Chemical and ACAPHA [J].

Gao, Guanghua ;

Law, Francis C. P. .

DRUG METABOLISM AND DISPOSITION, 2009, 37 (04) :884-891

[5] Medical progress: Idiopathic pulmonary fibrosis. [J].

Gross, TJ ;

Hunninghake, GW .

NEW ENGLAND JOURNAL OF MEDICINE, 2001, 345 (07) :517-525

[6] A data integration methodology for systems biology: Experimental verification [J].

Hwang, D ;

Smith, JJ ;

Leslie, DM ;

Weston, AD ;

Rust, AG ;

Ramsey, S ;

Atauri, PD ;

Siegel, AF ;

Bolouri, H ;

Aitchison, JD ;

Hood, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (48) :17302-17307

[7]

Jack A, 1990, CHEST, V97, P1439

[8] Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells [J].

Jiang, Hua ;

Hou, ChunHui ;

Zhang, ShuBing ;

Xie, HengYue ;

Zhou, WeiYing ;

Jin, QiHuang ;

Cheng, XiaoDong ;

Qian, RuoLan ;

Zhang, XueJun .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2007, 559 (2-3) :98-108

[9]

Jiang YY, 2010, CHIN J RESP CRIT CAR, V9, P44

[10]

Kaminski Naftali, 2006, Proc Am Thorac Soc, V3, P339, DOI 10.1513/pats.200601-011TK

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