Overexpression and characterization of a novel (S)-specific extended short-chain dehydrogenase/reductase from Candida parapsilosis

被引：21

作者：

Wang, Qiuyan ^{[1
]}

Shen, Linghong ^{[1
]}

Ye, Tingting ^{[1
]}

Cao, Dan ^{[1
]}

Chen, Rong ^{[1
]}

Pei, Xiaolin ^{[1
]}

Xie, Tian ^{[1
]}

Li, Yan ^{[2
]}

Gong, Weibo ^{[2
]}

Yin, Xiaopu ^{[1
]}

机构：

[1] Hangzhou Normal Univ, Coll Life & Environm Sci, Res Ctr Biomed & Hlth, Hangzhou 310012, Zhejiang, Peoples R China

[2] Meihua Holdings Grp Co Ltd, R&D Ctr, Langfang 065000, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2012年 / 123卷

基金：

中国国家自然科学基金;

关键词：

Extended short-chain dehydrogenase reductase; Asymmetric reduction; Chiral alcohol; DEPENDENT CARBONYL REDUCTASE; PURE ETHYL (S)-4-CHLORO-3-HYDROXYBUTANOATE; ESCHERICHIA-COLI; PICHIA-STIPITIS; PURIFICATION; DEHYDROGENASES/REDUCTASES; 4-CHLORO-3-OXOBUTANOATE; GENE; SDR;

D O I：

10.1016/j.biortech.2012.07.060

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The gene encoding a putative protein from Candida parapsilosis CDC317 (CPE) was cloned and overexpressed in Escherichia coli. The protein was amenable to overexpression in E. coli and constituted 35% of the total cell protein content. The optimal activity was determined at pH 5.5 and 40 degrees C with the substrate 4-chloro-3-oxobutanoate ethyl ester (COBE). The optical purity of the product was over 99% enantiomeric excess for the (S)-isomer, and the molar conversion yield of the product was 91.1%. The apparent k(m) value for CUBE was 0.19 +/- 0.01 mM, which is an order of magnitude lower than that of other enzymes in the literature. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：690 / 694

页数：5

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