Comparison of two methods for measurement of equine adrenocorticotropin

被引：12

作者：

Banse, Heidi E. ^{[1
]}

Schultz, Nichol ^{[2
]}

McCue, Molly ^{[2
]}

Geor, Ray ^{[3
]}

McFarlane, Dianne ^{[4
]}

机构：

[1] Univ Calgary, Dept Vet Clin & Diagnost Sci, Fac Vet Med, Calgary, AB, Canada

[2] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA

[3] Massey Univ, Coll Sci, Manawatu, New Zealand

[4] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Physiol Sci, Stillwater, OK 74078 USA

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2018年 / 30卷 / 02期

关键词：

ACTH; chemiluminescent immunoassay; horses; radioimmunoassay; PARS-INTERMEDIA DYSFUNCTION; HEALTHY HORSES; CUSHINGS-DISEASE; CLINICAL SIGNS; PITUITARY; HORMONE; PLASMA; ACTH;

D O I：

10.1177/1040638717752216

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Accurate measurement of equine adrenocorticotropin (ACTH) is important for the diagnosis of equine pituitary pars intermedia dysfunction (PPID). Several radioimmunoassays (RIAs) and chemiluminescent immunoassays (CIAs) are used for measurement of ACTH concentration in horses; whether these methods yield similar results across a range of concentrations is not determined. We evaluated agreement between a commercial RIA and CIA. Archived plasma samples (n = 633) were measured with both assays. Correlation between the 2 methods was moderate (r = 0.49, p < 0.001). Bland-Altman analysis revealed poor agreement, with a proportional bias and widening limits of agreement with increasing values. Poor agreement between assays was also observed when evaluating plasma samples with concentrations at or below the recommended diagnostic cutoff value for PPID testing. The lack of agreement suggests that measurements obtained should not be considered interchangeable between methods.

引用

页码：233 / 237

页数：5

共 18 条

[1] Adrenocorticotropin concentration following administration of thyrotropin-releasing hormone in healthy horses and those with pituitary pars intermedia dysfunction and pituitary gland hyperplasia [J].

Beech, Jill ;

Boston, Raymond ;

Lindborg, Sue ;

Russell, Gail E. .

JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2007, 231 (03) :417-426

[2] The tissue-specific processing of pro-opiomelanocortin [J].

Bicknell, A. B. .

JOURNAL OF NEUROENDOCRINOLOGY, 2008, 20 (06) :692-699

[3]

Bland JM, 1999, STAT METHODS MED RES, V8, P135, DOI 10.1177/096228029900800204

[4] Circannual variation in plasma adrenocorticotropic hormone concentrations in the UK in normal horses and ponies, and those with pituitary pars intermedia dysfunction [J].

Copas, V. E. N. ;

Durham, A. E. .

EQUINE VETERINARY JOURNAL, 2012, 44 (04) :440-443

[5] Circadian and circannual rhythms of cortisol, ACTH, and α-melanocyte-stimulating hormone in healthy horses [J].

Cordero, M. ;

Brorsen, B. W. ;

McFarlane, D. .

DOMESTIC ANIMAL ENDOCRINOLOGY, 2012, 43 (04) :317-324

[6] Plasma adrenocorticotropin concentration in healthy horses and in horses with clinical signs of hyperadrenocorticism [J].

Couetil, L ;

Paradis, MR ;

Knoll, J .

JOURNAL OF VETERINARY INTERNAL MEDICINE, 1996, 10 (01) :1-6

[7] Pituitary pars intermedia dysfunction: Diagnosis and treatment [J].

Durham, A. E. ;

McGowan, C. M. ;

Fey, K. ;

Tamzali, Y. ;

van der Kolk, J. H. .

EQUINE VETERINARY EDUCATION, 2014, 26 (04) :216-223

[8] EFFECT OF DOPAMINE ON THE SECRETION AND PROCESSING OF BETA-CELL TROPIN FROM ISOLATED PITUITARY NEUROINTERMEDIATE LOBES OF MICE [J].

EAGLE, L ;

MORTON, J ;

DUNMORE, S .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1993, 680 :499-501

[9] Assessment of ACTH assay variability: a multicenter study [J].

Giraldi, Francesca Pecori ;

Saccani, Andrea ;

Cavagnini, Francesco .

EUROPEAN JOURNAL OF ENDOCRINOLOGY, 2011, 164 (04) :505-512

[10] Photoperiod-Dependent Regulation of Carboxypeptidase E Affects the Selective Processing of Neuropeptides in the Seasonal Siberian Hamster (Phodopus sungorus) [J].

Helwig, M. ;

Herwig, A. ;

Heldmaier, G. ;

Barrett, P. ;

Mercer, J. G. ;

Klingenspor, M. .

JOURNAL OF NEUROENDOCRINOLOGY, 2013, 25 (02) :190-197

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