Phytochemical analysis of Pathyashadangam kwath and its standardization by HPLC and HPTLC

Background: Pathyashadangam kwath , a classical ayurvedic polyherbal formulation is used for the treatment of cluster head ache, migraine, upper respiratory diseases, ear ache and night blindness. Re- view of literature suggested that characterization parameters of Pathyashadangam kwath are not reported. Objective: To report characteristic parameters of Pathyashadangam kwath to con firm quality and purity. Materials and methods: The fruit pericarps of Haritaki, Bibhitaki and Amalaki , aerial parts of Bhunimba , rhizome of Haridra , stem bark of Nimba and stem of Guduchi were the ingredients of Pathyashadangam kwath . Three batches of the kwath were prepared as per standard procedures. The kwath was evaluated for organoleptic, physical, phytochemical and chromatographic parameters as per standard methods. Results: HPTLC analysis revealed that Toluene: Ethyl Acetate: Formic acid (2.5: 2.0: 0.5) was a suitable mobile phase for characterization of the kwath . HPLC analysis revealed that andrographolide was a suitable marker for standardization of the kwath . Conclusion: The characterization parameters presented in this paper may serve as standard reference for quality control analysis of Pathyashadangam kwath . (C) 2018 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Publishing Services by Elsevier B.V. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).