Phylogenetically diverse TT virus viremia among pregnant women

被引：21

作者：

Bzhalava, Davit ^{[1
,2
,3
,4
]}

Ekstrom, Johanna ^{[5
]}

Lysholm, Fredrik ^{[6
]}

Hultin, Emilie ^{[1
,2
,3
,4
]}

Faust, Helena ^{[5
]}

Persson, Bengt ^{[6
]}

Lehtinen, Matti ^{[7
]}

de Villiers, Ethel-Michele ^{[8
]}

Dinner, Joakim ^{[1
,2
,3
,4
,5
]}

机构：

[1] Karolinska Inst, Dept Lab Med, S-17177 Stockholm, Sweden

[2] Karolinska Inst, Dept Med Epidemiol, S-17177 Stockholm, Sweden

[3] Karolinska Inst, Dept Biostat, S-17177 Stockholm, Sweden

[4] Karolinska Univ Hosp, S-17177 Stockholm, Sweden

[5] Lund Univ, Dept Lab Med, Malmo, Sweden

[6] Linkoping Univ, IFM Bioinformat & SeRC Swedish E Sci Res Ctr, S-58183 Linkoping, Sweden

[7] Natl Inst Hlth & Welf, Oulu, Finland

[8] Deutsch Krebsforschungszentrum, Abt Tumorvirus Charakterisierung, D-69120 Heidelberg, Germany

来源：

VIROLOGY | 2012年 / 432卷 / 02期

基金：

瑞典研究理事会;

关键词：

Childhood leukemia; TT virus; High throughput sequencing; INTRAGENOMIC REARRANGEMENT; HODGKINS-DISEASE; DNA GENOME; INFECTION; GENERATION; IDENTIFICATION; PRIMATES; PROTEIN; FAMILY; MODEL;

D O I：

10.1016/j.virol.2012.06.022

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Infections during pregnancy have been suggested to be involved in childhood leukemias. We used high-throughput sequencing to describe the viruses most readily detectable in serum samples of pregnant women. Serum DNA of 112 mothers to leukemic children was amplified using whole genome amplification. Sequencing identified one TT virus (TTV) isolate belonging to a known type and two putatively new TTVs. For 22 mothers, we also performed ITV amplification by general primer PCR before sequencing. This detected 39 TTVs, two of which were identical to the Tilts found after whole genome amplification. Altogether, we found 40 TTV isolates, 29 of which were putatively new types (similarities ranging from 89% to 69%). In conclusion, high throughput sequencing is useful to describe the known or unknown viruses that are present in serum samples of pregnant women. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：427 / 434

页数：8

共 52 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].