Diversity and biological activities of the bacterial community associated with the marine sponge Phorbas tenacior (Porifera, Demospongiae)

被引:19
作者
Dupont, S. [1 ]
Carre-Mlouka, A. [1 ]
Descarrega, F. [1 ]
Ereskovsky, A. [2 ]
Longeon, A. [1 ]
Mouray, E. [1 ]
Florent, I. [1 ]
Bourguet-Kondracki, M. L. [1 ]
机构
[1] Museum Natl Hist Nat, Lab Mol Commun & Adaptat Microorganismes, UMR CNRS 7245, F-75005 Paris, France
[2] Aix Marseille Univ, Inst Mediterran Biodiversite & Ecol Marine & Cont, UMR CNRS 7263, Marseille, France
关键词
antimicrobial activity; antioxidant activity; antiplasmodial activity; associated bacteria; Phorbas tenacior; phylogenetic diversity; RAPD; sponge; SP-NOV; VERTICAL TRANSMISSION; ELECTRON-MICROSCOPE; DITERPENES; INSIGHTS;
D O I
10.1111/lam.12154
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The diversity of the cultivable microbiota of the marine sponge Phorbas tenacior frequently found in the Mediterranean Sea was investigated, and its potential as a source of antimicrobial, antioxidant and antiplasmodial compounds was evaluated. The cultivable bacterial community was studied by isolation, cultivation and 16S rRNA gene sequencing. Twenty-three bacterial strains were isolated and identified in the Proteobacteria ( or classes) and Actinobacteria phyla. Furthermore, three different bacterial morphotypes localized extracellularly within the sponge tissues were revealed by microscopic observations. Bacterial strains were assigned to seven different genera, namely Vibrio, Photobacterium, Shewanella, Pseudomonas, Ruegeria, Pseudovibrio and Citricoccus. The strains affiliated to the same genus were differentiated according to their genetic dissimilarities using random amplified polymorphic DNA (RAPD) analyses. Eleven bacterial strains were selected for evaluation of their bioactivities. Three isolates Pseudovibrio P1Ma4, Vibrio P1MaNal1 and Citricoccus P1S7 revealed antimicrobial activity; Citricoccus P1S7 and Vibrio P1MaNal1 isolates also exhibited antiplasmodial activity, while two Vibrio isolates P1Ma8 and P1Ma5 displayed antioxidant activity. These data confirmed the importance of Proteobacteria and Actinobacteria associated with marine sponges as a reservoir of bioactive compounds.
引用
收藏
页码:42 / 52
页数:11
相关论文
共 53 条
  • [51] NITROGEN-FIXATION IN CORAL-REEF SPONGES WITH SYMBIOTIC CYANOBACTERIA
    WILKINSON, CR
    FAY, P
    [J]. NATURE, 1979, 279 (5713) : 527 - 529
  • [52] Shewanella spongiae sp nov., isolated from a marine sponge
    Yang, Sung-Hyun
    Kwon, Kae Kyoung
    Lee, Hee-Soon
    Kim, Sang-Jin
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 : 2879 - 2882
  • [53] Phorbasins G-K: new cytotoxic diterpenes from a southern Australian marine sponge, Phorbas sp.
    Zhang, Hua
    Major, Jodie M.
    Lewis, Richard J.
    Capon, Robert J.
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2008, 6 (20) : 3811 - 3815