Novel coumarin-based containing denrons selective fluorescent chemosesor for sequential recognition of Cu2+ and PPi

In this study, we have successfully synthesized a novel coumarin-based dendrons derivative CD and its chemical structure was characterized by H-1 NMR, C-13 NMR and ESI-HR-MS. The sensor CD showed an obvious "on-off' fluorescence quenching response toward Cu2+ with a maximum quenching efficiency of 99.8%. The CD-Cu2+ complex showed an "off-on" fluorescence enhancement response toward PPi over many competitive anions. The detection limit of the sensor CD was 0.29 x 10(-6) M to Cu2+ and 239 x 10-9M to PPi. In addition, the sensor CD showed a 1:1 binding stoichiometry to Cu2+ and the sensor CD-Cu2+ showed a 2:1 binding stoichiometry to PPi in CH3CN/HEPES buffer medium (9:1 v/v, pH = 7.2). The stable pH range of sensor CD to Cu2+ and CD-Cu2+ to PPi was from 3 to 8. (C) 2019 Elsevier Ltd. All rights reserved.