Optimizing cell viability in droplet-based cell deposition

被引：92

作者：

Hendriks, Jan ^{[1
]}

Visser, Claas Willem ^{[2
]}

Henke, Sieger ^{[1
]}

Leijten, Jeroen ^{[1
]}

Saris, Daniel B. F. ^{[3
,4
]}

Sun, Chao ^{[2
]}

Lohse, Detlef ^{[2
]}

Karperien, Marcel ^{[1
]}

机构：

[1] Univ Twente, Dept Dev BioEngn, MIRA Inst Biomed Technol & Tech Med, NL-7500 AE Enschede, Netherlands

[2] Univ Twente, MIRA Inst Biomed Technol & Tech Med, Phys Fluids Grp, NL-7500 AE Enschede, Netherlands

[3] UMC Utrecht, Dept Orthoped, Utrecht, Netherlands

[4] Univ Twente, Dept Reconstruct Med, MIRA Inst Biomed Technol & Tech Med, Fac Sci & Technol, NL-7500 AE Enschede, Netherlands

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

IMPACT; KERATINOCYTES; TISSUES; BIOFABRICATION; DEFORMATION; SUSPENSION; DELIVERY;

D O I：

10.1038/srep11304

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Biofabrication commonly involves the use of liquid droplets to transport cells to the printed structure. However, the viability of the cells after impact is poorly controlled and understood, hampering applications including cell spraying, inkjet bioprinting, and laser-assisted cell transfer. Here, we present an analytical model describing the cell viability after impact as a function of the cell-surrounding droplet characteristics. The model connects (1) the cell survival as a function of cell membrane elongation, (2) the membrane elongation as a function of the cell-containing droplet size and velocity, and (3) the substrate properties. The model is validated by cell viability measurements in cell spraying, which is a method for biofabrication and used for the treatment of burn wounds. The results allow for rational optimization of any droplet-based cell deposition technology, and we include practical suggestions to improve the cell viability in cell spraying.

引用

页数：10

共 50 条

[1] Spray-on-skin cells in burns: A common practice with no agreed protocol [J].