Plexin-neuropilin-1 complexes form functional semaphorin-3A receptors

被引：703

作者：

Takahashi, T

Fournier, A

Nakamura, F

Wang, LH

Murakami, Y

Kalb, RG

Fujisawa, H

Strittmatter, SM ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Neurol, New Haven, CT 06510 USA

[2] Yale Univ, Sch Med, Neurobiol Sect, New Haven, CT 06510 USA

[3] Yale Univ, Sch Med, Dept Mol Cellular & Dev Biol, New Haven, CT 06510 USA

[4] Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA

[5] Nagoya Univ, Grad Sch Sci, Grp Dev Neurobiol, Div Biol Sci, Nagoya, Aichi 4648602, Japan

来源：

CELL | 1999年 / 99卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(00)80062-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Class 1 and 3 semaphorins repulse axons but bind to different cell surface proteins. We find that the two known semaphorin-binding proteins, plexin 1 (Plex 1) and neuropilin-1 (NP-1), form a stable complex. Plex 1 alone does not bind semaphorin-3A (Sema3A), but the NP-1/Plex 1 complex has a higher affinity for Sema3A than does NP-1 alone. While Sema3A binding to NP-1 does not alter nonneuronal cell morphology, Sema3A interaction with NP-1/Plex 1 complexes induces adherent cells to round up. Expression of a dominant-negative Plex 1 in sensory neurons blocks Sema3A-induced growth cone collapse. Sema3A treatment leads to the redistribution of growth cone NP-1 and plexin into clusters. Thus, physiologic Sema3A receptors consist of NP-1/plexin complexes.

引用

页码：59 / 69

页数：11

共 32 条

[1] Semaphorin III Is needed for normal patterning and growth of nerves, bones and heart [J].