Nanostructured selenium-doped biphasic calcium phosphate with in situ incorporation of silver for antibacterial applications

被引：33

作者：

Nie, Lei ^{[1
,2
,3
]}

Hou, Mengjuan ^{[1
]}

Wang, Tianwen ^{[1
]}

Sun, Meng ^{[1
]}

Hou, Ruixia ^{[4
]}

机构：

[1] Xinyang Normal Univ XYNU, Coll Life Sci, Xinyang 464000, Peoples R China

[2] Katholieke Univ Leuven, KU Leuven, Dept Mech Engn, B-3001 Leuven, Belgium

[3] Katholieke Univ Leuven, KU Leuven, Flanders Make, B-3001 Leuven, Belgium

[4] Ningbo Univ, Med Sch, Ningbo 315211, Peoples R China

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

基金：

中国国家自然科学基金;

关键词：

SUBSTITUTED HYDROXYAPATITE; MULTIFUNCTIONAL PROPERTIES; NANOPARTICLES; SCAFFOLDS; COMPOSITE; COATINGS; DIFFERENTIATION; REGENERATION; PARTICLES; TOXICITY;

D O I：

10.1038/s41598-020-70776-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Selenium-doped nanostructure has been considered as an attractive approach to enhance the antibacterial activity of calcium phosphate (CaP) materials in diverse medical applications. In this study, the selenium-doped biphasic calcium phosphate nanoparticles (SeB-NPs) were first synthesized. Then, silver was in situ incorporated into SeB-NPs to obtain nanostructured composite nanoparticles (AgSeB-NPs). Both SeB-NPs and AgSeB-NPs were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), X-ray photoelectron spectroscopy (XPS), and Raman spectra. The results confirmed that the SeO32- was doped at the PO43- position and silver nanoparticles were deposited on the surface of SeB-NPs. Next, Transmission Electron Microscopy (TEM) analysis displayed that the prepared AgSeB-NPs had a needle-cluster-like morphology. CCK-8 analysis revealed SeB-NPs and AgSeB-NPs had good cytocompatibility with osteoblasts. The antibacterial activity of the prepared AgSeB-NPs was confirmed by using Gram-negative E. coli and Gram-positive S. aureus. The above results manifested the significance of the final AgSeB-NPs for biomedical applications.

引用

页数：14

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