DNA methylation analysis by MethyLight technology

被引：105
作者：
Trinh, BN
Long, TI
Laird, PW
机构：
[1] Univ So Calif, Keck Sch Med, Kenneth Norris Jr Comprehens Canc Ctr, Dept Surg, Los Angeles, CA 90089 USA
[2] Univ So Calif, Keck Sch Med, Kenneth Norris Jr Comprehens Canc Ctr, Dept Biochem & Mol Biol, Los Angeles, CA 90089 USA
来源：
METHODS | 2001年 / 25卷 / 04期
关键词：
CpG island; hypermethylation; DNA methylation; Taqman; real-time PCR; sodium bisulfite;
D O I：
10.1006/meth.2001.1268
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
MethyLight is a sensitive, fluorescence-based real-time PCR technique that is capable of quantitating DNA methylation at a particular locus by using DNA oligonucleotides that anneal differentially to bisulfite-converted DNA according to the methylation status In the original genomic DNA. The use of three oligonucleotides (forward and reverse primers, and interpositioned probe) in MethyLight, any one or more of which can be used for methylation discrimination, allows for a high degree of specificity, sensitivity, and flexibility In methylation detection. (C) 2001 Elsevier Science (USA).
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收藏
页码：456 / 462
页数：7
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