Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae

被引：22
|
作者：
Dumke, Roger [1 ]
Jacobs, Enno [1 ]
机构：
[1] Tech Univ Dresden, Med Fac Carl Gustav Carus, Inst Med Microbiol & Hyg, D-01307 Dresden, Germany
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2009年 / 47卷 / 02期
关键词：
P1 CYTADHESIN GENE; CHLAMYDOPHILA-PNEUMONIAE; LEGIONELLA-PNEUMOPHILA; CHLAMYDIA-PNEUMONIAE; RESPIRATORY SAMPLES; CLINICAL STRAINS; INFECTIONS; SEQUENCE; MANIFESTATIONS; EPIDEMIOLOGY;
D O I：
10.1128/JCM.01989-08
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae. All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/mu l, but the mean crossing points resulted in differences in the concentration of the genome copies of a factor of 20.
引用
收藏
页码：441 / 444
页数：4
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