Micellar electrokinetic capillary chromatography as an alternative method for the determination of ethinylestradiol and levo-norgestrel

被引：21

作者：

Berzas, JJ ^{[1
]}

Del Castillo, B

Castañeda, G

Pinilla, MJ

机构：

[1] Univ Castilla La Mancha, Dept Quim Analit & Tecnol Alimentos, Fac Quim, E-13071 Ciudad Real, Spain

[2] Univ Complutense Madrid, Fac Farm, Secc Dept Quim Analit, Madrid, Spain

来源：

TALANTA | 1999年 / 50卷 / 02期

关键词：

ethinylestradiol; levo-norgestrel; micellar electrokinetic chromatography (MEKC); oral contraceptives;

D O I：

10.1016/S0039-9140(99)00030-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for quantifying of ethinylestradiol (ETE) and levo-norgestrel (LEV) in pharmaceutical products by micellar electrokinetic chromatography (MEKC) is described. The separation was carried out at 25 degrees C and 25 kV, using a 20 mM berate buffer (pH 9.2), 15 mM sodium dodecylsulfate (SDS) in 30% acetonitrile/water (v/v). Under these conditions the analysis takes about 7 min. The method has been applied for quantifying both compounds in six different commercial contraceptives and the proposed method gave good results when compared with a reference liquid chromatographic (LC) method. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：261 / 268

页数：8

共 22 条

[1] APPROACHES TO OPTIMIZATION OF PRECISION IN CAPILLARY ELECTROPHORESIS [J].