Potential Antiviral Lignans from the Roots of Saururus chinensis with Activity against Epstein-Barr Virus Lytic Replication

被引：59
作者：
Cui, Hui [1 ]
Xu, Bo [2 ]
Wu, Taizong [1 ]
Xu, Jun [1 ]
Yuan, Yan [2 ,3 ]
Gu, Qiong [1 ]
机构：
[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Res Ctr Drug Discovery, Guangzhou 510006, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Zhongshan Sch Med, Inst Human Virol, Guangzhou 510080, Guangdong, Peoples R China
[3] Univ Penn, Sch Dent Med, Dept Microbiol, Philadelphia, PA 19104 USA
来源：
JOURNAL OF NATURAL PRODUCTS | 2014年 / 77卷 / 01期
基金：
中国国家自然科学基金;
关键词：
NASOPHARYNGEAL CARCINOMA; TETRAHYDROFURAN LIGNANS; ABSOLUTE-CONFIGURATION; INHIBITORY-ACTIVITY; PIPER-SOLMSIANUM; ACTIVATION; NEOLIGNANS; SESQUILIGNANS; CONSTITUENTS; MANASSANTIN;
D O I：
10.1021/np400757k
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Epstein-Barr virus (EBV) is a member of the gamma-herpes virus subfamily and has been implicated in the pathogenesis of several human malignancies. Bioassay-guided fractionation was conducted on an EtOAc-soluble extract of the roots of Saururus chinensis and monitored using an EBV lytic replication assay. This led to the isolation of 19 new (1-19) and nine known (20-28) lignans. The absolute configurations of the new lignans were established by Mosher's ester, ECD, and computational methods. Eight lignans, including three sesquineolignans (19, 23, and 24) and five dineolignans (3, 4, 26, 27, and 28), exhibited inhibitory effects toward EBV lytic replication with EC50 values from 1.09 to 7.55 mu M and SI values from 3.3 to 116.4. In particular, manassantin B (27) exhibited the most promising inhibition, with an EC50 of 1.72 mu M, low cytotoxicity, CC50 > 200 mu M, and SI > 116.4. This is the first study demonstrating that lignans possess anti-EBV lytic replication activity.
引用
收藏
页码：100 / 110
页数：11
相关论文
共 36 条
[1]  
Bezerra AD, 2012, QUIM NOVA, V35, P2226, DOI 10.1590/S0100-40422012001100024
[2]   Inhibition of the Epstein-Barr virus lytic cycle by Zta-targeted RNA interference [J].
Chang, Y ;
Chang, SS ;
Lee, HH ;
Doong, SL ;
Takada, K ;
Tsai, CH .
JOURNAL OF GENERAL VIROLOGY, 2004, 85 :1371-1379
[3]   Cytotoxic Sesquilignans from the Roots of Saururus chinensis [J].
Chin, Young-Won ;
Cai, Xing-Fu ;
Ahn, Kyung-Seop ;
Lee, Hyeong-Kyu ;
Oh, Sei-Ryang .
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2010, 31 (07) :2088-2090
[4]   AN EBV GENOME CARRYING PRE-B CELL LEUKEMIA IN A HOMOSEXUAL MAN WITH CHARACTERISTIC KARYOTYPE AND IMPAIRED EBV-SPECIFIC IMMUNITY [J].
ERNBERG, I ;
BJORKHOLM, M ;
ZECH, L ;
SANDSTEDT, B ;
SZIGETI, R ;
ANDERSSON, J ;
HENLE, W ;
KLEIN, G .
JOURNAL OF CLINICAL ONCOLOGY, 1986, 4 (10) :1481-1488
[5]   Infraspecific variation of sulfur-containing bisamides from Aglaia leptantha [J].
Greger, H ;
Pacher, T ;
Vajrodaya, S ;
Bacher, M ;
Hofer, O .
JOURNAL OF NATURAL PRODUCTS, 2000, 63 (05) :616-620
[6]   A new benzofuranone and anti-HIV constituents from the stems of Rhus chinensis [J].
Gu, Qiong ;
Wang, Rui-Rui ;
Zhang, Xue-Mei ;
Wang, Yun-Hua ;
Zheng, Yong-Tang ;
Zhou, Jun ;
Chen, Ji-Jun .
PLANTA MEDICA, 2007, 73 (03) :279-282
[7]   Chrysanolide A, an unprecedented sesquiterpenoid trimer from the flowers of Chrysanthemum indicum L [J].
Gu, Qiong ;
Chen, Yaoyao ;
Cui, Hui ;
Huang, Dane ;
Zhou, Jingwei ;
Wu, Taizong ;
Chen, Yiping ;
Shi, Lina ;
Xu, Jun .
RSC ADVANCES, 2013, 3 (26) :10168-10172
[8]   CONSTITUENTS OF MACE .3. NEW NEOLIGNANS AND LIGNANS FROM THE ARIL OF MYRISTICA-FRAGRANS [J].
HADA, S ;
HATTORI, M ;
TEZUKA, Y ;
KIKUCHI, T ;
NAMBA, T .
PHYTOCHEMISTRY, 1988, 27 (02) :563-568
[9]   Total synthesis and stereochemical confirmation of manassantin A, B, and B1 [J].
Hanessian, Stephen ;
Reddy, Gone Jayapal ;
Chahal, Navjot .
ORGANIC LETTERS, 2006, 8 (24) :5477-5480
[10]   Epstein-Barr Virus LF2 Protein Regulates Viral Replication by Altering Rta Subcellular Localization [J].
Heilmann, Andreas M. F. ;
Calderwood, Michael A. ;
Johannsen, Eric .
JOURNAL OF VIROLOGY, 2010, 84 (19) :9920-9931
1234