Anatomical correlations of intrinsic axon repair after partial optic nerve crush in rats

被引:21
作者
Hanke, J [1 ]
机构
[1] Otto Von Guericke Univ, Fac Med, Inst Med Psychol, D-39120 Magdeburg, Germany
关键词
diffuse axonal injury; impaired axoplasmic transport; cytoskeletal change; retinal ganglion cells;
D O I
10.1016/S0940-9602(02)80002-4
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
About 15% of retinal ganglion cells survive diffuse axonal injury of the optic nerve in adult rats. Following initial blindness, discrimination of visual stimuli in behavioral tests recovers within three weeks. To investigate the mechanisms promoting this functional recovery the axonal transport and the neurofilaments were studied. Intraocularly applied MiniRuby is transported until the place of crush and accumulated in enlarged axon terminals. Three weeks after lesion the anterograde transport of MiniRuby recovers distal to the place of crush. At the same point in time the retrograde transport of surviving retinal ganglion cells is restored which was visualized by horseradish peroxidase injected into the superior colliculus. The heavy neurofilament was stained immunohistochemically and analyzed statistically up to three weeks after optic nerve crush. The stained filaments in the axon fibers of retinal ganglion cells appear wavelike and/or fragmented up to day 8, but first signs of heavy neurofilament restitution in the fibers of the optic nerve are seen at day 12 after axonal injury. Because these results cannot be explained by longlasting axon regeneration, the present results provide convincing evidence for intrinsic axon repair soon after diffuse axonal injury that correlates in time with recovery of vision.
引用
收藏
页码:113 / 123
页数:11
相关论文
共 46 条
[1]   Apoptotic versus necrotic characteristics of retinal ganglion cell death after partial optic nerve injury [J].
Bien, A ;
Seidenbecher, CI ;
Böckers, TM ;
Sabel, BA ;
Kreutz, MR .
JOURNAL OF NEUROTRAUMA, 1999, 16 (02) :153-163
[2]   TOPOGRAPHY OF AXONAL INJURY AS DEFINED BY AMYLOID PRECURSOR PROTEIN AND THE SECTOR SCORING METHOD IN MILD AND SEVERE CLOSED-HEAD INJURY [J].
BLUMBERGS, PC ;
SCOTT, G ;
MANAVIS, J ;
WAINWRIGHT, H ;
SIMPSON, DA .
JOURNAL OF NEUROTRAUMA, 1995, 12 (04) :565-572
[3]   Summary report: Evidence for the effectiveness of rehabilitation for persons with traumatic brain injury [J].
Chesnut, RM ;
Carney, N ;
Maynard, H ;
Mann, NC ;
Patterson, P ;
Helfand, M .
JOURNAL OF HEAD TRAUMA REHABILITATION, 1999, 14 (02) :176-188
[4]   DEFECTIVE AXONAL-TRANSPORT IN A TRANSGENIC MOUSE MODEL OF AMYOTROPHIC-LATERAL-SCLEROSIS [J].
COLLARD, JF ;
COTE, F ;
JULIEN, JP .
NATURE, 1995, 375 (6526) :61-64
[5]  
DUVDEVANI R, 1990, RESTOR NEUROL NEUROS, V2, P31, DOI 10.3233/RNN-1990-2104
[6]  
Gennarelli T.A., 1986, MECHANISMS SECONDARY, P15
[7]   AXONAL INJURY IN THE OPTIC-NERVE - A MODEL SIMULATING DIFFUSE AXONAL INJURY IN THE BRAIN [J].
GENNARELLI, TA ;
THIBAULT, LE ;
TIPPERMAN, R ;
TOMEI, G ;
SERGOT, R ;
BROWN, M ;
MAXWELL, WL ;
GRAHAM, DI ;
ADAMS, JH ;
IRVINE, A ;
GENNARELLI, LM ;
DUHAIME, AC ;
BOOCK, R ;
GREENBERG, J .
JOURNAL OF NEUROSURGERY, 1989, 71 (02) :244-253
[8]  
GENTLEMAN SM, 1995, ACTA NEUROPATHOL, V89, P537
[9]  
GRILL RJ, 1999, CNS REGENERATION BAS, P27