(-)-Epicatechin gallate prevents alkali-salt mediated fibrillogenesis of hen egg white lysozyme

被引：56

作者：

Ghosh, Sudeshna ^{[1
]}

Pandey, Nitin K. ^{[1
]}

Dasgupta, Swagata ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Kharagpur 721302, W Bengal, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 54卷

关键词：

Fibrillation; Hen egg white lysozyme; (-)-Epicatechin gallate (ECG); Inhibition; AMYLOID FIBRIL FORMATION; MEROZOITE SURFACE PROTEIN-2; HUMAN SERUM-ALBUMIN; GREEN TEA; (-)-EPIGALLOCATECHIN GALLATE; ALZHEIMERS-DISEASE; CIRCULAR-DICHROISM; TERTIARY BUTANOL; BETA OLIGOMERS; IN-VIVO;

D O I：

10.1016/j.ijbiomac.2012.11.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Green tea polyphenols (GTPs) are found to be potent inhibitors of amyloid fibril formation. We report the effective inhibitory property of (-)-epicatechin gallate (ECG) during the alkali-salt induced fibrillogenesis of hen egg white lysozyme (HEWL) at 37 degrees C. Spectroscopic techniques such as fluorescence, circular dichroism and microscopic images show that (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), and (-)-epigallocatechin gallate (EGCG) show moderate inhibition of fibrillation with ECG as the most potent polyphenol. Aromatic interactions, hydrophobic interactions, the radical scavenging activity and autoxidation of polyphenols are likely to be the major reasons for ECG being the most effective inhibitor. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：90 / 98

页数：9

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