Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation

被引：422
作者：
Joshi, AA [1 ]
Struhl, K [1 ]
机构：
[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
来源：
MOLECULAR CELL | 2005年 / 20卷 / 06期
关键词：
D O I：
10.1016/j.molcel.2005.11.021
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Eaf3, a component of the NuA4 histone acetylase and Rpd3 histone deacetylase complexes, is important for the global pattern of histone acetylation in Saccharomyces cerevisiae. Preferential deacetylation of coding regions requires the Eaf3 chromodomain and H3-K36 methylation by Set2. The Eaf3 chromodomain interacts with methylated H3-K36 peptides, suggesting that this interaction leads to preferential association and histone deacetylation of the 3' portions of coding regions by the Rpd3 complex. However, the Eaf3 chromodomain and H3-K36 methylation do not significantly affect acetylation at promoters, suggesting that Eaf3 has a distinct function, presumably in the NuA4 complex. Lastly, Eaf3 inhibits internal initiation within mRNA coding regions in a manner similar to FACT and Spt6. Our results link the pattern of preferential deacetylation at coding regions to the underlying patterns of H3-K36 methylation and phosphorylation of the RNA polymerase II C-terminal domain, and ultimately to the mechanism by which repressive chromatin structure is restored after transcriptional elongation.
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收藏
页码：971 / 978
页数：8
相关论文
共 28 条
  • [1] Aparicio O, 2004, CURRENT PROTOCOLS MO
  • [2] Global nucleosome occupancy in yeast
    Bernstein, BE
    Liu, CL
    Humphrey, EL
    Perlstein, EO
    Schreiber, SL
    [J]. GENOME BIOLOGY, 2004, 5 (09)
  • [3] Conservation of the MORF4 related gene family:: identification of a new chromo domain subfamily and novel protein motif
    Bertram, MJ
    Pereira-Smith, OM
    [J]. GENE, 2001, 266 (1-2) : 111 - 121
  • [4] The many colours of chromodomains
    Brehm, A
    Tufteland, KR
    Aasland, R
    Becker, PB
    [J]. BIOESSAYS, 2004, 26 (02) : 133 - 140
  • [5] Histone H3 methylation by Set2 directs deacetylation of coding regions by Rpd3S to suppress spurious intragenic transcription
    Carrozza, MJ
    Li, B
    Florens, L
    Suganuma, T
    Swanson, SK
    Lee, KK
    Shia, WJ
    Anderson, S
    Yates, J
    Washburn, MP
    Workman, JL
    [J]. CELL, 2005, 123 (04) : 581 - 592
  • [6] Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters
    Kadosh, D
    Struhl, K
    [J]. CELL, 1997, 89 (03) : 365 - 371
  • [7] Transcription elongation factors repress transcription initiation from cryptic sites
    Kaplan, CD
    Laprade, L
    Winston, F
    [J]. SCIENCE, 2003, 301 (5636) : 1096 - 1099
  • [8] Cotranscriptional Set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex
    Keogh, MC
    Kurdistani, SK
    Morris, SA
    Ahn, SH
    Podolny, V
    Collins, SR
    Schuldiner, M
    Chin, KY
    Punna, T
    Thompson, NJ
    Boone, C
    Emili, A
    Weissman, JS
    Hughes, TR
    Strahl, BD
    Grunstein, M
    Greenblatt, JF
    Buratowski, S
    Krogan, NJ
    [J]. CELL, 2005, 123 (04) : 593 - 605
  • [9] A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3K36 methylation with transcript elongation
    Kizer, KO
    Phatnani, HP
    Shibata, Y
    Hall, H
    Greenleaf, AL
    Strahl, BD
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (08) : 3305 - 3316
  • [10] Evidence for distinct mechanisms facilitating transcript elongation through chromatin in vivo
    Kristjuhan, A
    Svejstrup, JQ
    [J]. EMBO JOURNAL, 2004, 23 (21) : 4243 - 4252
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