Selective tumor antigen vaccine delivery to human CD169+ antigen-presenting cells using ganglioside-liposomes

被引：71

作者：

Affandi, Alsya J. ^{[1
]}

Grabowska, Joanna ^{[1
]}

Olesek, Katarzyna ^{[1
]}

Venegas, Miguel Lopez ^{[1
,2
]}

Barbaria, Arnaud ^{[1
]}

Rodriguez, Ernesto ^{[1
]}

Mulder, Patrick P. G. ^{[1
]}

Pijffers, Helen J. ^{[1
]}

Ambrosini, Martino ^{[1
]}

Kalay, Hakan ^{[1
]}

O'Toole, Tom ^{[1
]}

Zwart, Eline S. ^{[1
,3
]}

Kazemier, Geert ^{[3
]}

Nazmi, Kamran ^{[4
,5
]}

Bikker, Floris J. ^{[4
,5
]}

Stoeckl, Johannes ^{[6
]}

van den Eertwegh, Alfons J. M. ^{[7
]}

de Gruijl, Tanja D. ^{[7
]}

Storm, Gert ^{[8
,9
]}

van Kooyk, Yvette ^{[1
,2
]}

den Haan, Joke M. M. ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Canc Ctr Amsterdam, Amsterdam Infect & Immun Inst, Dept Mol Cell Biol & Immunol,Amsterdam UMC, NL-1081 HZ Amsterdam, Netherlands

[2] DC4U, NL-3621 ZA Breukelen, Netherlands

[3] Vrije Univ Amsterdam, Canc Ctr Amsterdam, Dept Surg, Amsterdam UMC, NL-1081 HV Amsterdam, Netherlands

[4] Vrije Univ Amsterdam, Acad Ctr Dent Amsterdam ACTA, Dept Oral Biochem, NL-1081 LA Amsterdam, Netherlands

[5] Univ Amsterdam, NL-1081 LA Amsterdam, Netherlands

[6] Med Univ Vienna, Ctr Pathophysiol Infectiol & Immunol, Inst Immunol, A-1090 Vienna, Austria

[7] Vrije Univ Amsterdam, Canc Ctr Amsterdam, Dept Med Oncol, Amsterdam UMC, NL-1081 HV Amsterdam, Netherlands

[8] Univ Utrecht, Utrecht Inst Pharmaceut Sci, Dept Pharmaceut, NL-3508 TB Utrecht, Netherlands

[9] Univ Twente, Fac Sci & Technol, Dept Biomat Sci & Technol, NL-7522 NB Enschede, Netherlands

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2020年 / 117卷 / 44期

基金：

欧盟地平线“2020”;

关键词：

Siglec-1; CD169; dendritic cells; CD8(+) T cells; vaccination; ACCELERATED BLOOD CLEARANCE; TARGETING DC-SIGN; DENDRITIC CELLS; TRANS-INFECTION; HUMAN SPLEEN; MACROPHAGES; SIALOADHESIN; BINDING; SPECIFICITIES; EXPRESSION;

D O I：

10.1073/pnas.2006186117

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Priming of CD8(+) T cells by dendritic cells (DCs) is crucial for the generation of effective antitumor immune responses. Here, we describe a liposomal vaccine carrier that delivers tumor antigens to human CD169/Siglec-1(+) antigen-presenting cells using gangliosides as targeting ligands. Ganglioside-liposomes specifically bound to CD169 and were internalized by in vitro-generated monocyte-derived DCs (moDCs) and macrophages and by ex vivo-isolated splenic macrophages in a CD169-dependent manner. In blood, high-dimensional reduction analysis revealed that ganglioside-liposomes specifically targeted CD14(+) CD169(+) monocytes and Axl(+) CD169(+) DCs. Liposomal codelivery of tumor antigen and Toll-like receptor ligand to CD169(+) moDCs and Axl(+) CD169(+) DCs led to cytokine production and robust cross-presentation and activation of tumor antigen-specific CD8(+) T cells. Finally, Axl(+) CD169(+) DCs were present in cancer patients and efficiently captured ganglioside-liposomes. Our findings demonstrate a nanovaccine platform targeting CD169(+) DCs to drive antitumor T cell responses.

引用

页码：27528 / 27539

页数：12

共 83 条

[1] Virus Particle Release from Glycosphingolipid-Enriched Microdomains Is Essential for Dendritic Cell-Mediated Capture and Transfer of HIV-1 and Henipavirus [J].

Akiyama, Hisashi ;

Miller, Caitlin ;

Patel, Hiren V. ;

Hatch, Steven C. ;

Archer, Jacob ;

Ramirez, Nora-Guadalupe P. ;

Gummuluru, Suryaram .

JOURNAL OF VIROLOGY, 2014, 88 (16) :8813-8825

[2] High-Dimensional Phenotypic Mapping of Human Dendritic Cells Reveals Interindividual Variation and Tissue Specialization [J].

Alcantara-Hernandez, Marcela ;

Leylek, Rebecca ;

Wagar, Lisa E. ;

Engleman, Edgar G. ;

Keler, Tibor ;

Marinkovich, M. Peter ;

Davis, Mark M. ;

Nolan, Garry P. ;

Idoyaga, Juliana .

IMMUNITY, 2017, 47 (06) :1037-+

[3] Diversification of human plasmacytoid predendritic cells in response to a single stimulus [J].

Alculumbre, Solana G. ;

Saint-Andre, Violaine ;

Di Domizio, Jeremy ;

Vargas, Pablo ;

Sirven, Philemon ;

Bost, Pierre ;

Maurin, Mathieu ;

Maiuri, Paolo ;

Wery, Maxime ;

San Roman, Mabel ;

Savey, Lea ;

Touzot, Maxime ;

Terrier, Benjamin ;

Saadoun, David ;

Conrad, Curdin ;

Gilliet, Michel ;

Morillon, Antonin ;

Soumelis, Vassili .

NATURE IMMUNOLOGY, 2018, 19 (01) :63-+

[4] Clinical use of dendritic cells for cancer therapy [J].

Anguille, Sebastien ;

Smits, Evelien L. ;

Lion, Eva ;

van Tendeloo, Viggo F. ;

Berneman, Zwi N. .

LANCET ONCOLOGY, 2014, 15 (07) :E257-E267

[5] CD169-positive sinus macrophages in the lymph nodes determine bladder cancer prognosis [J].

Asano, Touko ;

Ohnishi, Koji ;

Shiota, Takuya ;

Motoshima, Takanobu ;

Sugiyama, Yutaka ;

Yatsuda, Junji ;

Kamba, Tomomi ;

Ishizaka, Kazuhiro ;

Komohara, Yoshihiro .

CANCER SCIENCE, 2018, 109 (05) :1723-1730

[6] Skin tests predict survival after autologous tumor cell vaccination in metastatic melanoma: Experience in 81 patients [J].

Baars, A ;

Claessen, AME ;

van den Eertwegh, AJM ;

Gall, HE ;

Stam, AGM ;

Meijer, S ;

Giaccone, G ;

Meijer, CJLM ;

Scheper, RJ ;

Wagstaff, J ;

Vermorken, JB ;

Pinedo, HM .

ANNALS OF ONCOLOGY, 2000, 11 (08) :965-970

[7] Effective collaboration between marginal metallophilic macrophages and CD8+ dendritic cells in the generation of cytotoxic T cells [J].

Backer, Ronald ;

Schwandt, Timo ;

Greuter, Mascha ;

Oosting, Marije ;

Jungerkes, Frank ;

Tuting, Thomas ;

Boon, Louis ;

O'Toole, Tom ;

Kraal, Georg ;

Limmer, Andreas ;

den Haan, Joke M. M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (01) :216-221

[8] Identification of unique neoantigen qualities in long-term survivors of pancreatic cancer [J].

Balachandran, Vinod P. ;

Luksza, Marta ;

Zhao, Julia N. ;

Makarov, Vladimir ;

Moral, John Alec ;

Remark, Romain ;

Herbst, Brian ;

Askan, Gokce ;

Bhanot, Umesh ;

Senbabaoglu, Yasin ;

Wells, Daniel K. ;

Cary, Charles Ian Ormsby ;

Grbovic-Huezo, Olivera ;

Attiyeh, Marc ;

Medina, Benjamin ;

Zhang, Jennifer ;

Loo, Jennifer ;

Saglimbeni, Joseph ;

Abu-Akeel, Mohsen ;

Zappasodi, Roberta ;

Riaz, Nadeem ;

Smoragiewicz, Martin ;

Kelley, Z. Larkin ;

Basturk, Olca ;

Goenen, Mithat ;

Levine, Arnold J. ;

Allen, Peter J. ;

Fearon, Douglas T. ;

Merad, Miriam ;

Gnjatic, Sacha ;

Iacobuzio-Donahue, Christine A. ;

Wolchok, Jedd D. ;

DeMatteo, Ronald P. ;

Chan, Timothy A. ;

Greenbaum, Benjamin D. ;

Merghoub, Taha ;

Leach, Steven D. .

NATURE, 2017, 551 (7681) :512-+

[9] CD169+ macrophages are sufficient for priming of CTLs with specificities left out by cross-priming dendritic cells [J].

Bernhard, Caroline A. ;

Ried, Christine ;

Kochanek, Stefan ;

Brocker, Thomas .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (17) :5461-5466

[10] The CD169 sialoadhesin molecule mediates cytotoxic. T-cell responses to tumour apoptotic vesicles [J].

Black, Lane V. C. ;

Saunderson, Sarah C. ;

Coutinho, Frazer P. ;

Muhsin-Sharafaldine, Morad-Remy ;

Damani, Tanvi T. ;

Dunn, Amy C. ;

McLellan, Alexander D. .

IMMUNOLOGY AND CELL BIOLOGY, 2016, 94 (05) :430-438

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