Vaccination of chicken embryos with escape mutants of La Sota Newcastle disease virus induces a protective immune response

被引：35

作者：

Mast, J ^{[1
]}

Nanbru, U ^{[1
]}

Decaesstecker, M ^{[1
]}

Lambrecht, B ^{[1
]}

Couvreur, B ^{[1
]}

Meulemans, G ^{[1
]}

van den Berg, T ^{[1
]}

机构：

[1] Vet & Agrochem Res Ctr, B-1180 Brussels, Belgium

来源：

VACCINE | 2006年 / 24卷 / 11期

关键词：

Newcastle disease virus; in ovo vaccination; escape mutants; immunoselection; HN glycoprotein; F glycoprotein;

D O I：

10.1016/j.vaccine.2005.10.020

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To reduce the embryonic pathogenicity of Newcastle disease virus (NDV), escape mutants of the La Sota strain were produced with selected monoclonal antibodies. Immunoselection resulted in the elimination of an epitope by single amino acid substitution (F and HN molecule) or in a conformational change (HN molecule). The embryonic pathogenicity of these escape mutants was reduced and their dose was optimised for in ovo vaccination. Because antibody responses and protection of in ovo vaccinated chicks were similar to controls vaccinated at hatch with the La Sota strain, immunoselection appears a valuable technique to produce attenuated NDV strains, which are candidate in ovo vaccines. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：1756 / 1765

页数：10

共 40 条

[1] ANTIGENIC MAPPING AND FUNCTIONAL-ANALYSIS OF THE F-PROTEIN OF NEWCASTLE-DISEASE VIRUS USING MONOCLONAL-ANTIBODIES [J].