Sequence conservation of glycerophosphodiester phosphodiesterase among Treponema pallidum strains

被引：33

作者：

Cameron, CE ^{[1
]}

Castro, C ^{[1
]}

Lukehart, SA ^{[1
]}

Van Voorhis, WC ^{[1
]}

机构：

[1] Univ Washington, Dept Med, Seattle, WA 98195 USA

来源：

INFECTION AND IMMUNITY | 1999年 / 67卷 / 06期

关键词：

D O I：

10.1128/IAI.67.6.3168-3170.1999

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Previous investigations have demonstrated that immunization with Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase significantly protects rabbits from subsequent treponeme challenge. In this report, we show that the glycerophosphodiester phosphodiesterase amino acid sequence is conserved among 12 strains from a total of five pathogenic treponemes. The invariant nature of this immunoprotective antigen makes it an attractive candidate for inclusion in a universal subunit vaccine against T. pallidum infection. In addition, these studies show a silent nucleotide substitution at position 579 of the gpd open reading frame which is consistently observed in the non-T. pallidum subsp. pallidum strains. This sequence alteration introduces a PleI restriction site in the nonsyphilis strains and thus allows genetic differentiation from T. pallidum subsp. pallidum strains.

引用

页码：3168 / 3170

页数：3

共 22 条

[1]

BORENSTEIN LA, 1988, J IMMUNOL, V140, P2415

[2] Function and protective capacity of Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase [J].