Ascorbate biosynthesis in Arabidopsis cell suspension culture

被引：134

作者：

Davey, MW ^{[1
]}

Gilot, C ^{[1
]}

Persiau, G ^{[1
]}

Ostergaard, J ^{[1
]}

Han, Y ^{[1
]}

Bauw, GC ^{[1
]}

Van Montagu, MC ^{[1
]}

机构：

[1] State Univ Ghent VIB, Dept Plantengenet, Genet Lab, B-9000 Ghent, Belgium

来源：

PLANT PHYSIOLOGY | 1999年 / 121卷 / 02期

关键词：

D O I：

10.1104/pp.121.2.535

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The biosynthesis of L-ascorbic acid (L-AA) in an Arabidopsis (L.) Heynh. cell suspension culture was studied by quantifying the effects of incubation with a range of potential biosynthetic precursors, analogs, and inhibitors on the intracellular levels of reduced and oxidized forms of L-AA. Our results support the recently published biosynthetic pathway of L-AA from L-galactose (G.L. Wheeler, M.A. Jones, N. Smirnoff [1998] Nature 393: 365-369), but suggest that Arabidopsis cell suspension culture simultaneously contains two other routes leading to L-AA. The possible physiological significance of these alternate routes is discussed.

引用

页码：535 / 543

页数：9

共 49 条

[1]

[Anonymous], 1980, CARBOHYDRATES STRUCT, DOI DOI 10.1016/B978-0-12-675403-2.50010-7

[2] Lycorine: A powerful inhibitor of L-galactono-gamma-lactone dehydrogenase activity [J].

Arrigoni, O ;

DeGara, L ;

Paciolla, C ;

Evidente, A ;

dePinto, MC ;

Liso, R .

JOURNAL OF PLANT PHYSIOLOGY, 1997, 150 (03) :362-364

[3] ASCORBATE SYSTEM IN PLANT DEVELOPMENT [J].

ARRIGONI, O .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1994, 26 (04) :407-419

[4] LYCORINE AS AN INHIBITOR OF ASCORBIC-ACID BIOSYNTHESIS [J].

ARRIGONI, O ;

LISO, RA ;

CALABRESE, G .

NATURE, 1975, 256 (5517) :513-514

[5] L-ASCORBIC ACID BIOSYNTHESIS IN HIGHER PLANTS FROM L-GULONO-1,4-LACTONE AND L-GALACTONO-1,4-LACTONE [J].

BAIG, MM ;

KELLY, S ;

LOEWUS, F .

PLANT PHYSIOLOGY, 1970, 46 (02) :277-+

[6] Ascorbate metabolism and its regulation in animals [J].

Banhegyi, G ;

Braun, L ;

Csala, M ;

Puskas, F ;

Mandl, J .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 23 (05) :793-803

[7] NOVEL O-D-GALACTURONOYL ESTERS IN THE PECTIC POLYSACCHARIDES OF SUSPENSION-CULTURED PLANT-CELLS [J].

BROWN, JA ;

FRY, SC .

PLANT PHYSIOLOGY, 1993, 103 (03) :993-999

[8] STRUCTURAL MODELS OF PRIMARY-CELL WALLS IN FLOWERING PLANTS - CONSISTENCY OF MOLECULAR-STRUCTURE WITH THE PHYSICAL-PROPERTIES OF THE WALLS DURING GROWTH [J].

CARPITA, NC ;

GIBEAUT, DM .

PLANT JOURNAL, 1993, 3 (01) :1-30

[9]

CITTERIO S, 1994, PHYSIOL PLANTARUM, V92, P601

[10] Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant [J].

Conklin, PL ;

Williams, EH ;

Last, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (18) :9970-9974

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