Identification of active compounds from Aurantii Immatri Pericarpium attenuating brain injury in a rat model of ischemia-reperfusion

被引：19

作者：

Yang, Eun-Ju ^{[1
]}

Lim, Sun Ha ^{[2
]}

Song, Kyung-Sik ^{[3
]}

Han, Hyung Soo ^{[4
]}

Lee, Jongwon ^{[2
]}

机构：

[1] Kyungpook Natl Univ, Res Team Dev Funct Hlth Food Mat BK21, Taegu 702701, South Korea

[2] Catholic Univ Daegu, Sch Med, Dept Biochem, Taegu 705718, South Korea

[3] Kyungpook Natl Univ, Pharmaceut Sci Res Inst, Coll Pharm, Taegu 702702, South Korea

[4] Kyungpook Natl Univ, Sch Med, Dept Physiol, Taegu 700422, South Korea

来源：

FOOD CHEMISTRY | 2013年 / 138卷 / 01期

关键词：

Aurantii Immatri Pericarpium; Nobiletin; Tangeretin; Ischemia-reperfusion; Cerebral infarction; CEREBRAL-ISCHEMIA; STROKE; NOBILETIN; SERUM;

D O I：

10.1016/j.foodchem.2012.09.137

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Ischemic stroke is caused by brain injury due to prolonged ischemia by occlusion of cerebral arteries. In this study, we isolated active compounds from an ethanol extract of Aurantii Immatri Pericarpium (HY5356). We first showed by DNA fragmentation assay that HY5356 improved human hepatocellular carcinoma cells (HepG2) under hypoxic conditions by inhibiting apoptosis. When HY5356 was fractionated with dichloromethane (MC), ethyl acetate (EA) and n-butanol (BU), the MC fraction improved cell viability at the lowest concentration (100 mu g/ml). Intraperitoneal injection of HY5356 (200 mg/kg) or the MC fraction (200 mg/kg) to rats prior to occlusion attenuated brain injury significantly in a rat model of ischemia-reperfusion. Adopting cell viability under hypoxic conditions as an activity screening system, we isolated nobiletin and tangeretin as active compounds. The results suggest that intake of Aurantii Immatri Pericarpium containing nobiletin and tangeretin as active compounds might be beneficial for preventing ischemic stroke. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：663 / 670

页数：8

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