cAMP-dependent protein kinase type RI is found in clusters in the rat detergent-insoluble neuronal fraction

被引：13

作者：

Mucignat-Caretta, C

Conti, V

Caretta, A

机构：

[1] Univ Padua, Dipartimento Anat & Fisiol Umana, I-35131 Padua, Italy

[2] Univ Parma, Ist Biochim Vet, I-43100 Parma, Italy

来源：

NEUROSCIENCE LETTERS | 1999年 / 273卷 / 01期

关键词：

protein kinase A; brain; fluorescent cyclic nucleotides; immunohistochemistry; rat; cytoskeleton;

D O I：

10.1016/S0304-3940(99)00597-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Different types of cAMP dependent regulatory subunits have been characterized in the mam malian brain: RI alpha and beta, RII alpha and beta. The subcellular distribution of RI subunits has been examined in the rat brain. Partial amino acid sequencing of tryptic fragments from the Triton insoluble pellet of the rat brain shows that cAMP dependent regulatory subunits type RI alpha are found in this fraction. Immunohistochemistry shows that Triton-insoluble RI subunits are concentrated to form clusters and this distribution is distinct from RII subunits. Immunohistochemistry and fluorescent cAMP labeling show that the clusters bind fluorescent cAMP analogues. These results suggest that the high local concentration of RI subunits can modulate cAMP distribution among different cellular compartments. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

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页码：1 / 4

页数：4

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