A magic triangle for experimental phasing of macromolecules

被引：66
|
作者：
Beck, Tobias [1 ]
Krasauskas, Andrius [1 ]
Gruene, Tim [1 ]
Sheldrick, George M. [1 ]
机构：
[1] Univ Gottingen, Dept Struct Chem, D-37077 Gottingen, Germany
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2008年 / 64卷
关键词：
D O I：
10.1107/S0907444908030266
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Obtaining phase information for the solution of macromolecular structures is still one of the bottlenecks in X-ray crystallography. 5-Amino-2,4,6-triiodoisophthalic acid (I3C), in which three covalently bound iodines form an equilateral triangle, was incorporated into proteins in order to obtain phases by singlewavelength anomalous dispersion (SAD). An improved binding capability compared with simple heavy-metal ions, ready availability, improved recognition of potential heavy-atom sites and low toxicity make I3C particularly suitable for experimental phasing.
引用
收藏
页码：1179 / 1182
页数：4
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