Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity

被引：51

作者：

Chen, Tingting ^{[1
,2
,3
]}

Yang, Qingchuan ^{[2
]}

Gruber, Margaret ^{[4
]}

Kang, Junmei ^{[2
]}

Sun, Yan ^{[1
]}

Ding, Wang ^{[2
]}

Zhang, Tiejun ^{[2
]}

Zhang, Xinquan ^{[3
]}

机构：

[1] China Agr Univ, Coll Anim Sci & Technol, Dept Grassland Sci, Beijing 100193, Peoples R China

[2] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China

[3] Sichuan Agr Univ, Anim Sci & Technol Coll, Dept Grassland Sci, Yaan 625014, Peoples R China

[4] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Alfalfa; Ethylene response factor gene; Expression analysis; Subcellular localization; Transgenic tobacco; Salt stress; TRANSCRIPTION FACTOR; ABSCISIC-ACID; DROUGHT TOLERANCE; OSMOTIC-STRESS; AP2; DOMAIN; OVEREXPRESSION; SALT; ARABIDOPSIS; FAMILY; ACCUMULATION;

D O I：

10.1007/s11033-011-1421-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene response factors (ERF) play crucial roles in plant development and response to stresses. Here, a novel cDNA fragment (MsERF8) encoding an ERF protein with an AP2 domain was isolated and characterized from alfalfa. The MsERF8 cDNA has an open reading frame of 603 bp and encodes a nuclear protein of 201 amino acids. Q-RT-PCR analysis revealed that MsERF8 was strongly enriched in roots and leaves compared with stems, flower buds and flowers of mature alfalfa plants. Bioinformatic analysis of the MsERF8 promoter indicated a number of elements associated with stress-related responses, and MsERF8 transcripts in alfalfa seedlings were induced by NaCl, PEG6000, Al-2(SO4)(3) and five different hormones. Expression of MsERF8 in transgenic tobacco plants resulted in higher tolerance to salinity than with non-transgenic plants. This data shows that MsERF8 is a gene which prevents or alleviates salinity damage and has strong potential to impart salt tolerance to other crop plants.

引用

页码：6067 / 6075

页数：9

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