Effects of dissolved oxygen on the biooxidation process of refractory gold ores

被引：16

作者：

Sun, Li-Xin ^{[1
]}

Zhang, Xu ^{[1
]}

Tan, Wen-Song ^{[1
]}

Zhu, Ming-Long ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2012年 / 114卷 / 05期

基金：

国家高技术研究发展计划(863计划);

关键词：

Refractory sulfide gold ores; Biooxidation process; Dissolved oxygen; Acidithiobacillus ferrooxidans; Stirred tank bioreactors; ACIDITHIOBACILLUS-FERROOXIDANS; FERROUS-IRON; PYRITE; OXIDATION; SOLIDS; RUSTICYANIN; GROWTH;

D O I：

10.1016/j.jbiosc.2012.06.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

While multiple theories exist regarding the effect of dissolved oxygen (DO) on the biooxidation of minerals, few studies have been performed the cellular or molecular scale (e.g., genetics) and the mechanism remains unclear. In this paper, the effects of DO concentration on the biooxidation process of refractory sulfide gold ores by Acidithiobacillus ferrooxidans were investigated in the experimental stirred tank bioreactors (STRs). The results indicated that higher biooxidation and cell growth rates were correlated with higher DO concentration. The biooxidation process was restricted at 1.2 ppm DO due to oxygen limitation. Furthermore, the effects of DO on cellular and molecular scale were studied for the first time. The results demonstrated that the oxygen uptake rate (OUR), the Fe2+ oxidation activity and the rus gene expression of A. ferrooxidans all increased with the DO concentration, which might be responsible for the increase of the biooxidation rates with the DO concentration. This study provides insight into the potential impact of molecular-level mechanisms of DO in the biooxidation process of minerals. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：531 / 536

页数：6

共 30 条

[1] DYNAMIC MEASUREMENT OF VOLUMETRIC OXYGEN TRANSFER COEFFICIENT IN FERMENTATION SYSTEMS [J].

BANDYOPA.B ;

HUMPHREY, AE .

BIOTECHNOLOGY AND BIOENGINEERING, 1967, 9 (04) :533-&

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] Role of pyrite in formation of hydroxyl radicals in coal: possible implications for human health [J].

Cohn, Corey A. ;

Laffers, Richard ;

Simon, Sanford R. ;

O'Riordan, Thomas ;

Schoonen, Martin A. A. .

PARTICLE AND FIBRE TOXICOLOGY, 2006, 3 (01)

[4] PURIFICATION AND SOME PROPERTIES OF RUSTICYANIN, A BLUE COPPER PROTEIN INVOLVED IN IRON(II) OXIDATION FROM THIOBACILLUS-FERROOXIDANS [J].

COX, JC ;

BOXER, DH .

BIOCHEMICAL JOURNAL, 1978, 174 (02) :497-502

[5] DETERMINATION OF IRON AND IRON-ALUMINUM MIXTURES BY TITRATION WITH EDTA [J].

DAVIS, DG ;

JACOBSEN, WR .

ANALYTICAL CHEMISTRY, 1960, 32 (02) :215-217

[6] Oxygen and carbon dioxide kinetic challenges for thermophilic mineral bioleaching processes [J].

de Kock, SH ;

Barnard, P ;

du Plessis, CA .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2004, 32 :273-275

[7] Effect of solids on viability of acidophilic bacteria [J].

Deveci, H .

MINERALS ENGINEERING, 2002, 15 (12) :1181-1189

[8]

Dew D.W., 1997, BIOMINING, P45, DOI DOI 10.1007/978-3-662-06111-4_3

[9] Bioleaching of a cobaltiferous pyrite: A continuous laboratory-scale study at high solids concentration [J].

dHugues, P ;

Cezac, P ;

Cabral, T ;

Battaglia, F ;

TruongMeyer, XM ;

Morin, D .

MINERALS ENGINEERING, 1997, 10 (05) :507-527

[10] The Use of Oxygen Uptake Rate to Monitor Discovery of Microbial and Enzymatic Biocatalysts [J].

Dumsday, Geoff J. ;

Ocal, Gunseli ;

Bridger, John S. ;

Zachariou, Michael .

BIOTECHNOLOGY AND BIOENGINEERING, 2009, 102 (03) :673-683

← 1 2 3 →