Magnetic Nanoparticles Interact and Pass an In Vitro Co-Culture Blood-Placenta Barrier Model

被引:31
作者
Mueller, Elena K. [1 ]
Graefe, Christine [1 ]
Wiekhorst, Frank [2 ]
Bergemann, Christian [3 ]
Weidner, Andreas [4 ]
Dutz, Silvio [4 ]
Clement, Joachim H. [1 ]
机构
[1] Jena Univ Hosp, Dept Hematol & Oncol, Klinikum 1, D-07747 Jena, Germany
[2] Phys Tech Bundesanstalt Berlin, Abbestr 2-12, D-10587 Berlin, Germany
[3] Chemicell GmbH, Eresburgstr 22-23, D-12103 Berlin, Germany
[4] Tech Univ Ilmenau, Inst Biomed Engn & Informat BMTI, Gustav Kirchhoff Str 2, D-98693 Ilmenau, Germany
关键词
superparamagnetic nanoparticles; BeWo cell line; primary placental-derived pericyte; co-culture; IRON-OXIDE NANOPARTICLES; TRANSPORT; CELLS; QUANTIFICATION; ENVIRONMENT; METABOLISM; TOXICITY; FUTURE;
D O I
10.3390/nano8020108
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Magnetic nanoparticles are interesting tools for biomedicine. Before application, critical prerequisites have to be fulfilled. An important issue is the contact and interaction with biological barriers such as the blood-placenta barrier. In order to study these processes in detail, suitable in vitro models are needed. For that purpose a blood-placenta barrier model based on the trophoblast-like cell line BeWo and primary placenta-derived pericytes was established. This model was characterized by molecular permeability, transepithelial electrical resistance and cell-cell-contact markers. Superparamagnetic iron oxide nanoparticles (SPIONs) with cationic, anionic or neutral surface charge were applied. The localization of the nanoparticles within the cells was illustrated by histochemistry. The time-dependent passage of the nanoparticles through the BeWo/pericyte barrier was measured by magnetic particle spectroscopy and atomic absorption spectroscopy. Cationically coated SPIONs exhibited the most extensive interaction with the BeWo cells and remained primarily in the BeWo/pericyte cell layer. In contrast, SPIONs with neutral and anionic surface charge were able to pass the cell layer to a higher extent and could be detected beyond the barrier after 24 h. This study showed that the mode of SPION interaction with and passage through the in vitro blood-placenta barrier model depends on the surface charge and the duration of treatment.
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页数:13
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