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Magnetic Nanoparticles Interact and Pass an In Vitro Co-Culture Blood-Placenta Barrier Model
被引:31
作者:
Mueller, Elena K.
[1
]
Graefe, Christine
[1
]
Wiekhorst, Frank
[2
]
Bergemann, Christian
[3
]
Weidner, Andreas
[4
]
Dutz, Silvio
[4
]
Clement, Joachim H.
[1
]
机构:
[1] Jena Univ Hosp, Dept Hematol & Oncol, Klinikum 1, D-07747 Jena, Germany
[2] Phys Tech Bundesanstalt Berlin, Abbestr 2-12, D-10587 Berlin, Germany
[3] Chemicell GmbH, Eresburgstr 22-23, D-12103 Berlin, Germany
[4] Tech Univ Ilmenau, Inst Biomed Engn & Informat BMTI, Gustav Kirchhoff Str 2, D-98693 Ilmenau, Germany
关键词:
superparamagnetic nanoparticles;
BeWo cell line;
primary placental-derived pericyte;
co-culture;
IRON-OXIDE NANOPARTICLES;
TRANSPORT;
CELLS;
QUANTIFICATION;
ENVIRONMENT;
METABOLISM;
TOXICITY;
FUTURE;
D O I:
10.3390/nano8020108
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
Magnetic nanoparticles are interesting tools for biomedicine. Before application, critical prerequisites have to be fulfilled. An important issue is the contact and interaction with biological barriers such as the blood-placenta barrier. In order to study these processes in detail, suitable in vitro models are needed. For that purpose a blood-placenta barrier model based on the trophoblast-like cell line BeWo and primary placenta-derived pericytes was established. This model was characterized by molecular permeability, transepithelial electrical resistance and cell-cell-contact markers. Superparamagnetic iron oxide nanoparticles (SPIONs) with cationic, anionic or neutral surface charge were applied. The localization of the nanoparticles within the cells was illustrated by histochemistry. The time-dependent passage of the nanoparticles through the BeWo/pericyte barrier was measured by magnetic particle spectroscopy and atomic absorption spectroscopy. Cationically coated SPIONs exhibited the most extensive interaction with the BeWo cells and remained primarily in the BeWo/pericyte cell layer. In contrast, SPIONs with neutral and anionic surface charge were able to pass the cell layer to a higher extent and could be detected beyond the barrier after 24 h. This study showed that the mode of SPION interaction with and passage through the in vitro blood-placenta barrier model depends on the surface charge and the duration of treatment.
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页数:13
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