Cellular Uptake of Covalent Poly(allylamine hydrochloride) Microcapsules and Its Influences on Cell Functions

被引：37
作者：
Wang, Bing [1 ]
Zhang, Yuying [1 ]
Mao, Zhengwei [1 ]
Gao, Changyou [1 ]
机构：
[1] Zhejiang Univ, MOE, Key Lab Macromol Synth & Functionalizat, Dept Polymer Sci & Engn, Hangzhou 310027, Zhejiang, Peoples R China
来源：
MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 11期
关键词：
cell functions; cell uptake; colloids; microcapsules; poly(allylamine hydrochloride); POLYELECTROLYTE MICROCAPSULES; POLYMER MICROCAPSULES; MOLECULAR-WEIGHT; COATED PIT; CAPSULES; PARTICLES; NANOPARTICLES; MIGRATION; RELEASE; INTERNALIZATION;
D O I：
10.1002/mabi.201200182
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The mechanism of the cellular uptake of polyelectrolyte microcapsules and its influences on the functions and toxicity of human SMCs are explored. The covalently assembled poly(allylamine hydrochloride)/glutaraldehyde microcapsules are easily ingested by SMCs mainly through macropinosis and caveolae-mediated endocytosis pathways. The capsules mainly disperse in cytoplasm without colocalization in early endosomes and cell nucleus. The results of gene chips reveal substantial and profound alternation of cell phenotypes and functions. Uptake of the microcapsules cause a slight decrease of cell viability, but leads to significant changes in cytoskeleton organization, cell cycle, as well as cell adhesion and migration ability.
引用
收藏
页码：1534 / 1545
页数：12
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