Genome-wide identification and comparative analysis of genes encoding AAPs in apple (Malus x domestica Borkh.)

被引：2
作者：
Feng, Zi-Quan [1 ]
Wang, Xun [1 ]
Li, Tong [1 ]
Wang, Xiao-Fei [1 ]
Li, Hui-Feng [2 ]
You, Chun-Xiang [1 ]
机构：
[1] Shandong Agr Univ, Natl Res Ctr Apple Engn & Technol, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
[2] Shandong Inst Pomol, Tai An 271018, Shandong, Peoples R China
来源：
GENE | 2022年 / 832卷
基金：
中国国家自然科学基金;
关键词：
Apple; Amino acid permease; Protein structure; Gene expression; AMINO-ACID TRANSPORTER; COMPATIBLE SOLUTE TRANSPORTERS; FUNCTIONAL-CHARACTERIZATION; SUBSTRATE-SPECIFICITY; EXPRESSION; PERMEASE; PREDICTION; FAMILY; TRANSCRIPTION; METABOLISM;
D O I：
10.1016/j.gene.2022.146558
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Amino acid permeases (AAPs) play important roles in plant amino acid transport and nitrogen metabolism. In this study, we carried a comprehensive analysis for apple genes encoding AAPs using bioinformatics and molecular biology. Eleven MdAAPs were identified by a genome-wide search and comparative genomic analysis revealed relatively conserved gene composition, transmembrane characteristics, and protein structures. Phylogenetic tree construction and analysis of the conserved motifs of MdAAPs and AtAAPs showed that AAPs can be classified into three groups (I, II, and III). We compared the promoters of the identified genes and did gene functional annotation and qRT-PCR and found a relationship between apple AAPs and nitrogen deficiency. The expression profile data implied that MdAAPs exhibit diversified distributions and functions in different tissues.
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页数：12
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