Enzymatic transglycosylation of PEG brushes by β-galactosidase

被引：13
作者：
Fang, Yan [1 ]
Xu, Wei [2 ]
Wu, Jian [2 ]
Xu, Zhi-Kang [1 ]
机构：
[1] Zhejiang Univ, Dept Polymer Sci & Engn, MOE Key Lab Macromol Synth & Functionalizat, Hangzhou 310027, Zhejiang, Peoples R China
[2] Zhejiang Univ, Dept Chem, Hangzhou 310027, Zhejiang, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 91期
基金：
中国国家自然科学基金;
关键词：
SELF-ASSEMBLED MONOLAYERS; CARBOHYDRATE MICROARRAYS; SUGAR ARRAYS; GLYCOSYLATION; ADSORPTION; SURFACE;
D O I：
10.1039/c2cc35369e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Enzymatic transglycosylation has been successfully implemented by beta-galactosidase (beta-Gal) on PEG brushes. Kinetic parameters validate this kind of chemoenzymatic strategy which is promising for construction of biomimetic surfaces with glycocalyx-like structures.
引用
收藏
页码：11208 / 11210
页数：3
相关论文
共 44 条
[1]   Encoded fiber-optic microsphere arrays for probing protein-carbohydrate interactions [J].
Adams, EW ;
Ueberfeld, J ;
Ratner, DM ;
O'Keefe, BR ;
Walt, DR ;
Seeberger, PH .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (43) :5317-5320
[2]   Saccharide display on microtiter plates [J].
Bryan, MC ;
Plettenburg, O ;
Sears, P ;
Rabuka, D ;
Wacowich-Sgarbi, S ;
Wong, CH .
CHEMISTRY & BIOLOGY, 2002, 9 (06) :713-720
[3]   Detection of Transglucosidase-Catalyzed Polysaccharide Synthesis on a Surface in Real Time Using Surface Plasmon Resonance Spectroscopy [J].
Cle, Carla ;
Gunning, A. Patrick ;
Syson, Karl ;
Bowater, Laura ;
Field, Robert A. ;
Bornemann, Stephen .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (46) :15234-+
[4]   Siglecs and their roles in the immune system [J].
Crocker, Paul R. ;
Paulson, James C. ;
Varki, Ajit .
NATURE REVIEWS IMMUNOLOGY, 2007, 7 (04) :255-266
[5]  
Disney M.D., 2004, Drug Discov. Today: TARGETS, V3, P151
[6]   Surface modification of a solid-state cellulose matrix with lactose by a surfactant-enveloped enzyme in a nonaqueous medium [J].
Egusa, Shizuka ;
Yokota, Shingo ;
Tanaka, Kyoko ;
Esaki, Kei ;
Okutani, Yuri ;
Ogawa, Yukiko ;
Kitaoka, Takuya ;
Goto, Masahiro ;
Wariishi, Hiroyuki .
JOURNAL OF MATERIALS CHEMISTRY, 2009, 19 (13) :1836-1842
[7]   Synthesis of sugar arrays in microtiter plate [J].
Fazio, F ;
Bryan, MC ;
Blixt, O ;
Paulson, JC ;
Wong, CH .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (48) :14397-14402
[8]   Carbohydrate microarrays - a new set of technologies at the frontiers of glycomics [J].
Feizi, T ;
Fazio, F ;
Chai, WC ;
Wong, CH .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2003, 13 (05) :637-645
[9]   Oligosaccharide microarrays for high-throughput detection and specificity assignments of carbohydrate-protein interactions [J].
Fukui, S ;
Feizi, T ;
Galustian, C ;
Lawson, AM ;
Chai, WG .
NATURE BIOTECHNOLOGY, 2002, 20 (10) :1011-1017
[10]   Comparison of coatings from reactive star shaped PEG-stat-PPG prepolymers and grafted linear PEG for biological and medical applications [J].
Groll, J ;
Ademovic, Z ;
Ameringer, T ;
Klee, D ;
Moeller, M .
BIOMACROMOLECULES, 2005, 6 (02) :956-962
12345